Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), University of Copenhagen, Copenhagen, Denmark.
Methods Mol Biol. 2024;2779:145-158. doi: 10.1007/978-1-0716-3738-8_8.
This chapter is an extension of the original publication by Schraivogel et al. (Science 375:315-320, 2022) which described, for the first time, image-enabled and high-speed cell sorting based on the BD CellView technology. It summarizes the technical aspects of the instrument in an easy-to-digest form and provides example-based guidance toward implementation of the CellView-based image cell sorting technology. As an example, it explains how to use the image-enabled cell sorter to analyze the chemically induced fragmentation of the Golgi apparatus in HeLa cells-an experiment that was alluded to in the original publication but was not included in the manuscript due to space constraints. The chemically induced Golgi fragmentation sort illustrates an elegant example of the utility of image-enabled cell sorting as a significant expansion of the single-cell toolbox. It is such a striking phenotype when analyzed with image cytometry but undetectable when using conventional flow cytometry. Described in a straightforward and concise manner, this experiment serves as a standard system assurance for image-based cell sorters.
这一章是 Schraivogel 等人原始出版物的扩展，该出版物首次描述了基于 BD CellView 技术的图像增强和高速细胞分选。它以易于理解的形式总结了仪器的技术方面，并提供了基于示例的指导，帮助实施基于 CellView 的图像细胞分选技术。作为一个示例，它解释了如何使用图像增强型细胞分选器来分析 HeLa 细胞中化学诱导的高尔基体碎裂 - 该实验在原始出版物中有所提及，但由于篇幅限制，未包含在论文中。化学诱导的高尔基体碎裂分选说明了图像增强型细胞分选的一个很好的例子，它是单细胞工具包的一个重要扩展。当用图像细胞术分析时，它是一个非常明显的表型，但当使用传统的流式细胞术时，它是无法检测到的。以简单明了的方式描述，该实验是图像细胞分选器的标准系统保证。
