Effects of Gene Deletion on Cell Division of Fission Yeast and Its Molecular Mechanism.

The gene encodes 60S ribosomal protein L10, which is involved in intracellular protein synthesis and cell growth. However, it is not yet known whether it is involved in the regulation of cell mitosis dynamics. This study focuses on the growth, spore production, cell morphology, the dynamics of microtubules, chromosomes, actin, myosin, and mitochondria of fission yeast () to investigate the impact of deletion on cell mitosis. RNA-Seq and bioinformatics analyses were also used to reveal key genes, such as , and , and proteasome pathways. The results showed that deletion resulted in slow cell growth, abnormal spore production, altered cell morphology, and abnormal microtubule number and length during interphase. The cell dynamics of the strain showed that the formation of a monopolar spindle leads to abnormal chromosome segregation with increased rate of spindle elongation in anaphase of mitosis, decreased total time of division, prolonged formation time of actin and myosin loops, and increased expression of mitochondrial proteins. Analysis of the RNA-Seq sequencing results showed that the proteasome pathway, up-regulation of , and down-regulation of and in the strain were the main factors underpinning the increased number of spore production. Also, in the strain, down-regulation of caused the abnormal microtubule and chromosome dynamics, and down-regulation of and were the key genes affecting the delay of actin ring and myosin ring formation. This study reveals the effect and molecular mechanism of gene deletion on cell division, which provides the scientific basis for further clarifying the function of the Rpl1001 protein in cell division.