Yu Wen, Yuan Rongmei, Liu Mengnan, Liu Ke, Ding Xiang, Hou Yiling
Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), College of Life Science, China West Normal University, Nanchong 637009, China.
College of Environmental Science and Engineering, China West Normal University, Nanchong 637009, China.
Curr Issues Mol Biol. 2024 Mar 18;46(3):2576-2597. doi: 10.3390/cimb46030164.
The gene encodes 60S ribosomal protein L10, which is involved in intracellular protein synthesis and cell growth. However, it is not yet known whether it is involved in the regulation of cell mitosis dynamics. This study focuses on the growth, spore production, cell morphology, the dynamics of microtubules, chromosomes, actin, myosin, and mitochondria of fission yeast () to investigate the impact of deletion on cell mitosis. RNA-Seq and bioinformatics analyses were also used to reveal key genes, such as , and , and proteasome pathways. The results showed that deletion resulted in slow cell growth, abnormal spore production, altered cell morphology, and abnormal microtubule number and length during interphase. The cell dynamics of the strain showed that the formation of a monopolar spindle leads to abnormal chromosome segregation with increased rate of spindle elongation in anaphase of mitosis, decreased total time of division, prolonged formation time of actin and myosin loops, and increased expression of mitochondrial proteins. Analysis of the RNA-Seq sequencing results showed that the proteasome pathway, up-regulation of , and down-regulation of and in the strain were the main factors underpinning the increased number of spore production. Also, in the strain, down-regulation of caused the abnormal microtubule and chromosome dynamics, and down-regulation of and were the key genes affecting the delay of actin ring and myosin ring formation. This study reveals the effect and molecular mechanism of gene deletion on cell division, which provides the scientific basis for further clarifying the function of the Rpl1001 protein in cell division.
该基因编码60S核糖体蛋白L10，其参与细胞内蛋白质合成和细胞生长。然而，尚不清楚它是否参与细胞有丝分裂动力学的调控。本研究聚焦于裂殖酵母（）的生长、孢子产生、细胞形态、微管、染色体、肌动蛋白、肌球蛋白和线粒体的动力学，以研究缺失对细胞有丝分裂的影响。还利用RNA测序和生物信息学分析来揭示关键基因，如、和以及蛋白酶体途径。结果表明，缺失导致细胞生长缓慢、孢子产生异常、细胞形态改变以及间期微管数量和长度异常。菌株的细胞动力学表明，单极纺锤体的形成导致有丝分裂后期染色体分离异常，纺锤体伸长速率增加，分裂总时间减少，肌动蛋白和肌球蛋白环的形成时间延长，线粒体蛋白表达增加。对RNA测序结果的分析表明，蛋白酶体途径、菌株中上调以及和下调是孢子产生数量增加的主要因素。此外，在菌株中，下调导致微管和染色体动力学异常，下调以及和是影响肌动蛋白环和肌球蛋白环形成延迟的关键基因。本研究揭示了基因缺失对细胞分裂的影响及其分子机制，为进一步阐明Rpl1001蛋白在细胞分裂中的功能提供了科学依据。
