Genome Analysis of a Newly Discovered Yeast Species, .

Annual surveys of Irish soil samples identified three isolates, CBS 16921 (UCD88), CBS 18246 (UCD443), and CBS 18247 (UCD483), of an apiculate yeast species within the genus. The internal transcribed spacer (ITS) and D1/D2 region of the large subunit (LSU) rRNA sequences showed that these are isolates of the recently described species , first isolated from Southwest China. No genome sequence for is currently available. The genome sequences of the three Irish isolates were determined using short-read (Illumina) sequencing, and the sequence of one isolate (CBS 16921) was assembled to chromosome level using long-read sequencing (Oxford Nanopore Technologies). Phylogenomic analysis shows that belongs to the fast-evolving lineage (FEL) of . Only one idiomorph (encoding α1) was identified in all three sequenced isolates, consistent with one mating type of a heterothallic species. Genome comparisons showed that there has been a rearrangement near α of FEL species compared to isolates from the slowly evolving lineage (SEL).