Lee Benjamin, Kader Md Abdul, Alam Masud, Dickson Dorothy M, Harvey Patrick, Colgate E Ross, Taniuchi Mami, Petri William A, Haque Rashidul, Kirkpatrick Beth D
Department of Pediatrics, Vaccine Testing Center and Translational Global Infectious Diseases Research Center, Larner College of Medicine, University of Vermont, Burlington, VT 05405, USA.
International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka 1212, Bangladesh.
Pathogens. 2024 Mar 4;13(3):223. doi: 10.3390/pathogens13030223.
The infant non-secretor histoblood group antigen phenotype is associated with reduced risk of symptomatic rotavirus diarrhea, one of the leading global causes of severe pediatric diarrheal disease and mortality. However, little is known regarding the role of secretor status in asymptomatic rotavirus infections. Therefore, we performed a nested case-control study within a birth cohort study previously conducted in Dhaka, Bangladesh, to determine the association between infant secretor phenotype and the odds of asymptomatic rotavirus infection, in addition to the risk of rotavirus diarrhea, in unvaccinated infants. In the parent cohort, infants were enrolled in the first week of life and followed through the first two years of life with multiple clinic visits and active surveillance for diarrheal illness. Secretor phenotyping was performed on saliva. Eleven surveillance stools collected over the first year of life were tested for rotavirus by real-time RT-PCR, followed by conventional PCR and amplicon sequencing to identify the infecting P-type of positive specimens. Similar to findings for symptomatic diarrhea, infant non-secretors experienced significantly fewer primary episodes of asymptomatic rotavirus infection through the first year of life in a likely rotavirus P-genotype-dependent manner. These data suggest that non-secretors experienced reduced risk from rotavirus due to decreased susceptibility to infection rather than reduced infection severity.
婴儿非分泌型组织血型抗原表型与有症状轮状病毒腹泻风险降低相关，有症状轮状病毒腹泻是导致严重小儿腹泻病和死亡的主要全球病因之一。然而，关于分泌型状态在无症状轮状病毒感染中的作用，人们知之甚少。因此，我们在先前于孟加拉国达卡进行的一项出生队列研究中开展了一项巢式病例对照研究，以确定婴儿分泌型表型与未接种疫苗婴儿无症状轮状病毒感染几率之间的关联，以及轮状病毒腹泻风险。在母队列中，婴儿在出生第一周入组，并在生命的头两年接受多次门诊随访以及腹泻病主动监测。对唾液进行分泌型表型分析。在生命的第一年收集的11份监测粪便样本通过实时逆转录聚合酶链反应（RT-PCR）检测轮状病毒，随后进行常规PCR和扩增子测序，以鉴定阳性样本的感染P型。与有症状腹泻的研究结果相似，婴儿非分泌型在生命的第一年经历的无症状轮状病毒感染初发次数明显较少，且可能以轮状病毒P基因型依赖的方式出现。这些数据表明，非分泌型因对感染的易感性降低而非感染严重程度降低，从而轮状病毒风险降低。
