Kannan Jiji, Pang Ka-Lai, Ho Ying-Ning, Hsu Pang-Hung, Chen Li-Li
Institute of Marine Biology, National Taiwan Ocean University, Keelung 20224, Taiwan.
Department of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung 20224, Taiwan.
Antioxidants (Basel). 2024 Mar 11;13(3):336. doi: 10.3390/antiox13030336.
This study represents a primary investigation centered on screening six marine fungi, , , , , , and sp.7, associated with the red algae , which was collected from Chao-Jin Park in Keelung, Taiwan, as potential immunostimulants for shrimp aquaculture. Recognizing the imperative for novel strategies to combat pathogen resistance arising from the use of antibiotics and vaccines in aquaculture, this study aimed to evaluate the metabolomic profile, antioxidant capabilities, and antibacterial properties of marine fungi. The antibacterial activity of the fungal extract was evaluated against five major aquaculture pathogens: , , , , and . The viability and cytotoxicity of marine fungal extracts were preliminarily evaluated using brine shrimps before assessing cytotoxicity, growth performance, immune efficacy, and disease resistance in white shrimp. The present study demonstrated that total phytochemical analysis correlated with antioxidant activity. and exhibited the strongest DPPH antioxidant scavenging activities of half-maximal inhibitory concentration (IC50) 16.5 ± 1.2 and 12.2 ± 2.6, which are comparable to ascorbic acid. LC-HDMSE analysis of the marine fungal extracts identified more than 8000 metabolites mainly classified under the superclass level of organic oxygen compounds, Organoheterocyclic compounds, Phenylpropanoids and polyketides, alkaloid and derivatives, benzenoids, lignans and neolignans, lipid and lipid-like molecules, nucleotides and nucleosides, organic nitrogen compounds, and organic acids and derivatives. Overall, our study significantly contributes to the advancement of sustainable practices by exploring alternative antimicrobial solutions and harnessing the bioactive potential inherent in marine endophytic fungi. In conclusion, our study advances our comprehension of fungal communities and their applications and holds promise for the development of effective and environmentally friendly approaches for enhancing shrimp health and productivity.
本研究是一项初步调查，重点筛选了六种与红藻相关的海洋真菌，即[具体真菌名称1]、[具体真菌名称2]、[具体真菌名称3]、[具体真菌名称4]、[具体真菌名称5]和sp.7，这些红藻采自中国台湾基隆的潮境公园，旨在寻找可用于虾类养殖的潜在免疫刺激剂。鉴于水产养殖中使用抗生素和疫苗导致病原体产生耐药性，急需新的应对策略，本研究旨在评估海洋真菌的代谢组学特征、抗氧化能力和抗菌特性。评估了真菌提取物对五种主要水产养殖病原体的抗菌活性，这五种病原体分别是[具体病原体名称1]、[具体病原体名称2]、[具体病原体名称3]、[具体病原体名称4]和[具体病原体名称5]。在评估对白斑虾的细胞毒性、生长性能、免疫功效和抗病能力之前，先用卤虫对海洋真菌提取物的活力和细胞毒性进行了初步评估。本研究表明，总植物化学分析与抗氧化活性相关。[具体真菌名称1]和[具体真菌名称2]表现出最强的1,1-二苯基-2-三硝基苯肼（DPPH）抗氧化清除活性，半数抑制浓度（IC50）分别为16.5±1.2和12.2±2.6，与抗坏血酸相当。对海洋真菌提取物进行的液相色谱-高分辨质谱（LC-HDMSE）分析鉴定出8000多种代谢物，主要归类于有机氧化合物、有机杂环化合物、苯丙素和聚酮化合物、生物碱及其衍生物、苯类化合物、木脂素和新木脂素、脂质及类脂分子、核苷酸和核苷、有机氮化合物以及有机酸及其衍生物等超类水平。总体而言，我们的研究通过探索替代抗菌解决方案并利用海洋内生真菌固有的生物活性潜力，为可持续养殖实践的发展做出了重要贡献。总之，我们的研究增进了我们对真菌群落及其应用的理解，并有望开发出有效且环保的方法来提高虾的健康水平和生产力。
