Mërtiri Ilir, Păcularu-Burada Bogdan, Stănciuc Nicoleta
Faculty of Food Science and Engineering, Dunărea de Jos University of Galati, 111 Domnească Street, 800201 Galati, Romania.
Antioxidants (Basel). 2024 Mar 13;13(3):345. doi: 10.3390/antiox13030345.
This paper aims to investigate the phytochemical profile and in vitro antibacterial activity of two juniper species collected in Albania, and . The berries and the needle leaves were subjected to solid-liquid solvent ultrasound-assisted extraction. The phytochemical characterization of the extracts was performed by spectrophotometric and chromatographic means. The extract of berries (JcB) showed a higher total phenolic and flavonoid content (3.04 ± 0.09 mg GAE/g DW, and 1.14 ± 0.36 mg QE/g DW, respectively), also a higher antioxidant activity from DPPH and ABTS radical screening assays, compared to berries (JoxB) extract. The extract of needle leaves (JoxL) prevailed in total flavonoid content (10.55 ± 0.24 mg QE/g DW), and ABTS assays (1.83 ± 0.01 mM TE/g DW), compared to the extract of needle leaves (JcL). The chromatographic analysis revealed the presence of ellagic acid and kaempferol in all the samples. Ellagic acid was the main identified compound with the highest quantity in the extracts of JoxB, JoxL, and JcB with an average of 445.69 ± 0.96 µg/g, 2890.05 ± 0.29 µg/g, and 8133.83 ± 4.03 µg/g, respectively. The antibacterial potential of the ethanolic extracts was evaluated on spp., , and . In the Agar Well Diffusion Assay, it was observed that all the tested bacterial strains were sensitive to the extracts, whereas selected extracts showed a similar inhibition activity rate compared with the antibiotic substance (Chloramphenicol), used as a positive control. The extracts showed a similar minimal inhibitory and bactericidal concentration for the individual bacterial strains, suggesting that and extracts have a similar potential in antibacterial activity.
本文旨在研究在阿尔巴尼亚采集的两种杜松属植物的植物化学特征及体外抗菌活性。对其浆果和针叶进行固液溶剂超声辅助提取。采用分光光度法和色谱法对提取物进行植物化学表征。与刺柏浆果（JoxB）提取物相比，刺柏浆果（JcB）提取物的总酚和黄酮含量更高（分别为3.04±0.09毫克没食子酸当量/克干重和1.14±0.36毫克槲皮素当量/克干重），在DPPH和ABTS自由基筛选试验中也具有更高的抗氧化活性。与刺柏针叶（JcL）提取物相比，刺柏针叶（JoxL）提取物的总黄酮含量（10.55±0.24毫克槲皮素当量/克干重）和ABTS试验结果（1.83±0.01毫摩尔TE/克干重）更优。色谱分析表明，所有样品中均存在鞣花酸和山奈酚。鞣花酸是主要鉴定出的化合物，在JoxB、JoxL和JcB提取物中含量最高，平均分别为445.69±0.96微克/克、2890.05±0.29微克/克和8133.83±4.03微克/克。对乙醇提取物的抗菌潜力进行了针对金黄色葡萄球菌、大肠杆菌和枯草芽孢杆菌的评估。在琼脂扩散试验中，观察到所有受试菌株对提取物敏感，而所选提取物与用作阳性对照的抗生素物质（氯霉素）相比，显示出相似的抑制活性率。提取物对各菌株的最低抑菌浓度和杀菌浓度相似，表明刺柏和刺柏提取物在抗菌活性方面具有相似的潜力。
