Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China.
Kunpeng Institute of Modern Agriculture at Foshan, Agricultural Genomics Institute, Chinese Academy of Agricultural Sciences, Foshan 528226, China.
Genes (Basel). 2024 Feb 21;15(3):269. doi: 10.3390/genes15030269.
Skeletal muscle plays critical roles in providing a protein source and contributing to meat production. It is well known that microRNAs (miRNAs) exert important effects on various biological processes in muscle, including cell fate determination, muscle fiber morphology, and structure development. However, the role of miRNA in skeletal muscle development remains incompletely understood. In this study, we observed a critical miRNA, miR-24-3p, which exhibited higher expression levels in Tongcheng (obese-type) pigs compared to Landrace (lean-type) pigs. Furthermore, we found that miR-24-3p was highly expressed in the dorsal muscle of pigs and the quadriceps muscle of mice. Functionally, miR-24-3p was found to inhibit proliferation and promote differentiation in muscle cells. Additionally, miR-24-3p was shown to facilitate the conversion of slow muscle fibers to fast muscle fibers and influence the expression of , a glucose transporter. Moreover, in a mouse model of skeletal muscle injury, we demonstrated that overexpression of miR-24-3p promoted rapid myogenesis and contributed to skeletal muscle regeneration. Furthermore, miR-24-3p was found to regulate the expression of target genes, including , , , , and . Collectively, our findings provide evidence that miR-24-3p plays a regulatory role in myogenesis and fiber type conversion. These findings contribute to our understanding of human muscle health and have implications for improving meat production traits in livestock.
骨骼肌在提供蛋白质来源和促进肉类生产方面起着至关重要的作用。众所周知，microRNAs（miRNAs）对肌肉中的各种生物过程（包括细胞命运决定、肌纤维形态和结构发育）发挥着重要作用。然而，miRNA 在骨骼肌发育中的作用仍不完全清楚。在这项研究中，我们观察到了一个关键的 miRNA，miR-24-3p，它在通城（肥胖型）猪中的表达水平高于长白猪（瘦型）。此外，我们发现 miR-24-3p 在猪的背肌和鼠的四头肌中高度表达。功能上，miR-24-3p 被发现可抑制肌肉细胞的增殖并促进其分化。此外，miR-24-3p 可促进慢肌纤维向快肌纤维的转化，并影响葡萄糖转运蛋白 的表达。此外，在骨骼肌损伤的小鼠模型中，我们证明过表达 miR-24-3p 可促进快速肌生成，并有助于骨骼肌再生。此外，miR-24-3p 被发现可调节靶基因的表达，包括 、 、 、 和 。总之，我们的研究结果表明，miR-24-3p 在肌生成和纤维类型转换中发挥着调节作用。这些发现有助于我们了解人类肌肉健康，并对改善家畜的肉质生产性状具有重要意义。
