Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education & Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan 430070, China.
Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Livestock and Poultry Multi-Omics of MARA, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518000, China.
Int J Mol Sci. 2023 Mar 5;24(5):4995. doi: 10.3390/ijms24054995.
Skeletal muscle, as a regenerative organization, plays a vital role in physiological characteristics and homeostasis. However, the regulation mechanism of skeletal muscle regeneration is not entirely clear. miRNAs, as one of the regulatory factors, exert profound effects on regulating skeletal muscle regeneration and myogenesis. This study aimed to discover the regulatory function of important miRNA miR-200c-5p in skeletal muscle regeneration. In our study, miR-200c-5p increased at the early stage and peaked at first day during mouse skeletal muscle regeneration, which was also highly expressed in skeletal muscle of mouse tissue profile. Further, overexpression of miR-200c-5p promoted migration and inhibited differentiation of C2C12 myoblast, whereas inhibition of miR-200c-5p had the opposite effect. Bioinformatic analysis predicted that has potential binding sites for miR-200c-5p at 3'UTR region. Dual-luciferase and RIP assays further proved that is a target gene of miR-200c-5p. The expression patterns of miR-200c-5p and were opposite during the skeletal muscle regeneration. Moreover, miR-200c-5p can rescue the effects of in the C2C12 myoblast. In conclusion, miR-200c-5p might play a considerable function during skeletal muscle regeneration and myogenesis. These findings will provide a promising gene for promoting muscle health and candidate therapeutic target for skeletal muscle repair.
骨骼肌作为一种可再生组织，在生理特性和内稳态中起着至关重要的作用。然而，骨骼肌再生的调控机制尚不完全清楚。miRNA 作为调节因子之一，对调节骨骼肌再生和肌发生具有深远的影响。本研究旨在发现重要 miRNA miR-200c-5p 在骨骼肌再生中的调节功能。在我们的研究中，miR-200c-5p 在小鼠骨骼肌再生的早期阶段增加，并在第 1 天达到峰值，在小鼠组织图谱的骨骼肌中也高度表达。进一步研究表明，miR-200c-5p 的过表达促进 C2C12 成肌细胞的迁移并抑制其分化，而抑制 miR-200c-5p 则产生相反的效果。生物信息学分析预测，在 3'UTR 区域存在 miR-200c-5p 的潜在结合位点。双荧光素酶和 RIP 实验进一步证实了 是 miR-200c-5p 的靶基因。在骨骼肌再生过程中，miR-200c-5p 和 的表达模式相反。此外，miR-200c-5p 可以挽救 miR-200c-5p 在 C2C12 成肌细胞中的作用。综上所述，miR-200c-5p 在骨骼肌再生和肌发生过程中可能发挥重要作用。这些发现为促进肌肉健康提供了有前途的基因，并为骨骼肌修复提供了候选治疗靶点。
