Ren Yahui, Liang Yun, Zhu Tao, Fan Yanru, Zhang Sijin, Zheng Mengmeng, Xiao Xiaoxue, Cheng Qingmin, Liu Yue, Chen Hui, Song Wei
School of Life Science and Engineering, Henan University of Urban Construction, Pingdingshan, 467044 Henan China.
School of Life Science, Yangtze University, No.1 Xueyuan Road, Jingzhou, 434022 Hubei China.
Cytotechnology. 2025 Jun;77(3):101. doi: 10.1007/s10616-025-00766-y. Epub 2025 May 15.
Linn., a medicinal and food-homologous plant, is commonly used to resist bacterial, inflammatory, tumor, and other effects. This study aimed to elucidate the multi-target mechanism of action of Linn. on liver cancer through network pharmacological analysis and verify its effect through biological experiments. The network pharmacology and molecular docking results showed that there were 152 interactivity targets between Linn. and liver cancer, which were mainly enriched in various biological processes through the AKT and MAPK signaling pathways. In vitro biological experiments showed that isorhamnetin and quercetin, the main active components of Linn., had significant inhibitory effect on liver cancer cells HepG2. In addition, the expression of AKT and MEK proteins was downregulated, which proved that both isorhamnetin and quercetin promoted apoptosis by activating the AKT and MAPK signaling pathways. In summary, our findings clarify the inhibitory effect of the active ingredients of Linn. against HepG2 cells and provide inspiration for its clinical application in the treatment of liver cancer.
The online version contains supplementary material available at 10.1007/s10616-025-00766-y.
[植物名称] Linn. 是一种药食同源植物，常用于抵抗细菌、抗炎、抗肿瘤等。本研究旨在通过网络药理学分析阐明[植物名称] Linn. 对肝癌的多靶点作用机制，并通过生物学实验验证其效果。网络药理学和分子对接结果表明，[植物名称] Linn. 与肝癌之间存在152个相互作用靶点，主要通过AKT和MAPK信号通路富集于各种生物学过程。体外生物学实验表明，[植物名称] Linn. 的主要活性成分异鼠李素和槲皮素对肝癌细胞HepG2具有显著抑制作用。此外，AKT和MEK蛋白的表达下调，这证明异鼠李素和槲皮素均通过激活AKT和MAPK信号通路促进细胞凋亡。综上所述，我们的研究结果阐明了[植物名称] Linn. 的活性成分对HepG2细胞的抑制作用，并为其在肝癌治疗中的临床应用提供了启示。
在线版本包含可在10.1007/s10616-025-00766-y获取的补充材料。
