Grzywa-Czuba Renata, Trojanek Joanna Beata, Michałkiewicz Jacek, Kubiszewska Izabela, Obrycki Łukasz, Wierzbicka-Rucińska Aldona, Litwin Mieczysław
Department of Microbiology and Clinical Immunology, The Children's Memorial Health Institute, Al. Dzieci Polskich 20, 04-730 Warsaw, Poland.
Department of Immunology, Nicolaus Copernicus University Collegium Medicum, M. Sklodowskiej-Curie 9, 85-094 Bydgoszcz, Poland.
J Pers Med. 2024 Feb 28;14(3):255. doi: 10.3390/jpm14030255.
A decrease in IGF-1 is often linked to inflammation. Low systemic and local IGF-1 production and downregulation of IGF-1R expression may precede and predict PH development in children/adolescents. Leukocyte mRNA expression of IGF-1 and its receptor (IGF-1R) and plasma IGF-1 were measured in a group of 39 PH children/adolescents (29 boys and 10 girls) and 35 age-matched normotensive children (19 boys and 16 girls) using the RT-PCR and ELISA tests. The expression of the IGF-1R protein was assessed by flow cytometry. Plasma IGF-1 concentration was evaluated with ELISA. The expression of IGF-1 and IGF-1R and plasma concentrations of IGF-1 did not differ between groups. However, the PH children had a decreased percentage in IGF-1R-bearing lymphocytes ( = 0.02) and monocytes ( = 0.0003), as well as a low density of IGF-R in monocytes ( = 0.02). The IGF-1 expression was negatively correlated with pulse-wave velocity (PWV) (r = -0.49), systolic blood pressure (SBP) (-0.44), and carotid intima-media thickness (cIMT) (-0.43). The IGF-1R expression was negatively correlated with PWV (r = -0.42) and SBP (r = -0.41). Our results suggest that early subclinical hypertensive arterial injury is associated with lower activity of IGF-1-IGF-1R expression and loss of protective actions.
胰岛素样生长因子-1（IGF-1）水平降低常与炎症相关。儿童/青少年中，全身和局部IGF-1生成减少以及IGF-1受体（IGF-1R）表达下调可能先于并预示着肺动脉高压（PH）的发生。采用逆转录聚合酶链反应（RT-PCR）和酶联免疫吸附测定（ELISA）试验，对39例PH儿童/青少年（29例男孩和10例女孩）和35例年龄匹配的血压正常儿童（19例男孩和16例女孩）进行了白细胞中IGF-1及其受体（IGF-1R）的mRNA表达以及血浆IGF-1的检测。通过流式细胞术评估IGF-1R蛋白的表达。用ELISA评估血浆IGF-1浓度。两组间IGF-1和IGF-1R的表达以及IGF-1的血浆浓度无差异。然而，PH儿童中携带IGF-1R的淋巴细胞比例降低（P = 0.02），单核细胞比例降低（P = 0.0003），单核细胞中IGF-R密度也较低（P = 0.02）。IGF-1表达与脉搏波速度（PWV）呈负相关（r = -0.49）、与收缩压（SBP）呈负相关（r = -0.44）、与颈动脉内膜中层厚度（cIMT）呈负相关（r = -0.43）。IGF-1R表达与PWV呈负相关（r = -0.42）、与SBP呈负相关（r = -0.41）。我们的结果表明，早期亚临床高血压性动脉损伤与IGF-1 - IGF-1R表达活性降低及保护作用丧失有关。
