Xu Jiao, Kong Lingyang, Ren Weichao, Wang Zhen, Tang Lili, Wu Wei, Liu Xiubo, Ma Wei, Zhang Shuquan
Institute of economic crops, Heilongjiang Academy of Agricultural Sciences, Harbin, China.
College of Jiamusi, Heilongjiang University of Chinese Medicine, jiamusi, China.
Heliyon. 2024 Mar 16;10(6):e27817. doi: 10.1016/j.heliyon.2024.e27817. eCollection 2024 Mar 30.
Terpene synthases () regulate plant growth, development, and stress response. genes have been identified in and . genes were identified and analyzed using bioinformatics. Genomic data were downloaded from Plant Transcription Factor Database and National Center for Biotechnology Information database, and genes were predicted, analyzed, and visualized using ExPASy, PlantCare, and other online websites along with TBtools, MEGA software, and other software. To verify its role, quantitative real-time polymerase chain reaction (qRT-PCR) tests were conducted. The family comprises 41 elements distributed over 8 chromosomes and a single scaffold segment. The isoelectric point varied between 4.96 and 7.03, while the molecular weight spanned from 20705.90 to 102324.64 Da. The majority of genes were found in the cytoplasm and chloroplasts, with the remainder situated in the peroxisome, nucleus, plasma membrane, and mitochondria. Several -acting components associated with stress response were present in the gene's upstream promoter region. Data from RNA sequencing and qRT-PCR revealed specific expression of genes in all five organs of female plants. Collinearity analysis showed 4 homologous gene pairs between the and , with many pairs of homologous genes in other species, which was consistent with the dicotyledons evolutionary relationship. Furthermore, some genes may participate in growth and development and play a role in secondary metabolite synthesis. Therefore, bioinformatics analysis of the gene family provides a theoretical basis for future research on the volatile terpene compounds of .
萜类合酶调控植物的生长、发育和应激反应。已在[具体物种1]和[具体物种2]中鉴定出萜类合酶基因。利用生物信息学对萜类合酶基因进行了鉴定和分析。从植物转录因子数据库和美国国立生物技术信息中心数据库下载了基因组数据，并使用ExPASy、PlantCare等在线网站以及TBtools、MEGA软件等软件对萜类合酶基因进行了预测、分析和可视化。为验证其作用，进行了定量实时聚合酶链反应（qRT-PCR）检测。萜类合酶基因家族包含41个元件，分布在8条染色体和一个单支架片段上。等电点在4.96至7.03之间变化，而分子量范围为20705.90至102324.64道尔顿。大多数基因位于细胞质和叶绿体中，其余位于过氧化物酶体、细胞核、质膜和线粒体中。该基因的上游启动子区域存在几个与应激反应相关的顺式作用元件。RNA测序和qRT-PCR数据显示萜类合酶基因在雌性[具体植物名称]植株的所有五个器官中均有特异性表达。共线性分析表明[具体物种1]和[具体物种2]之间有4对同源基因，在其他物种中也有许多对同源基因，这与双子叶植物的进化关系一致。此外，一些基因可能参与[具体植物名称]的生长发育，并在次生代谢产物合成中发挥作用。因此，对萜类合酶基因家族的生物信息学分析为未来研究[具体植物名称]的挥发性萜类化合物提供了理论依据。
