Aslam Muhammad Rahil, Asif Hafiz Muhammad, Alamri Abdulaziz, Bhutto Adnan Ahmed, Mukhtiar Muhammad, Ahmad Khalil, Muhammad Ashfaq Mehak, Sattar Hafiz Abdul, Hayee Abdul, Jabbar Sana, Zahid Rabia, Nawaz Allah
University College of Conventional Medicine, Faculty of Medicine & Allied Health Sciences, The Islamia University of Bahawalpur, Bahawalpur, 63100, Pakistan.
First Department of Internal Medicine, Graduate School of Medical & Pharmaceutical Sciences, University of Toyama, Toyama, 930-0194, Japan.
Heliyon. 2024 Mar 15;10(6):e27279. doi: 10.1016/j.heliyon.2024.e27279. eCollection 2024 Mar 30.
() and () are widely found in flora of the Cholistan Desert of Bahawalpur, Pakistan and are traditionally used to treat pain and chronic disease. The current study aimed to evaluate their phytochemical screening, antioxidant activity, phagocytic activity, and analgesic activity. Both the plant extracts were investigated for phytochemical screening, Fourier Transform Infrared (FTIR) analysis, antioxidant by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method, immunomodulatory activity by macrophages phagocytosis using carbon clearance rate assay and analgesic activity by acetic acid produced writhing method. Phytochemical screening showed the presence of carbohydrates, saponins, tannins, phenols, quinines, proteins, terpenes, glycosides, and alkaloids. FTIR analysis revealed the existence of different functional groups in both extracts. The DPPH method showed that exhibited a high antioxidant potential with an IC of 62.5 μg/ml whereas showed a lower antioxidant potential. At the dose of 200 mg/kg body weight (b. wt), both the extracts showed a significant increase in the phagocytic index by 5.2 ± 0.2, and, 4.8 ± 0.1 (p < 0.001) respectively which was close to the 100 mg/kg b. wt of the standard drug (Levamisole) 5.4 ± 0.2. Both the extracts at the dose of 200 mg/kg b. wt also significantly reduced the writhing (abdominal contractions) count by 13.7 ± 0.3 and, 25.3 ± 1.5 ( < 0.001), showing 71.8% and 47.6% of reduced analgesic activity compared to the standard drug dicloran (diclofenac sodium), respectively. In conclusion, extracts of both plants indicate their role in the management of various disorders to relieve pain and modulate the immune system.
（）和（）在巴基斯坦巴哈瓦尔布尔乔利斯坦沙漠的植物群中广泛存在，传统上用于治疗疼痛和慢性病。当前的研究旨在评估它们的植物化学筛选、抗氧化活性、吞噬活性和镇痛活性。对两种植物提取物都进行了植物化学筛选、傅里叶变换红外（FTIR）分析、通过2,2-二苯基-1-苦基肼（DPPH）法测定抗氧化活性、使用碳清除率测定法通过巨噬细胞吞噬作用评估免疫调节活性以及通过乙酸诱导扭体法评估镇痛活性。植物化学筛选表明存在碳水化合物、皂苷、单宁、酚类、奎宁、蛋白质、萜类、糖苷和生物碱。FTIR分析揭示了两种提取物中存在不同的官能团。DPPH法表明，（提取物名称1）表现出高抗氧化潜力，IC50为62.5μg/ml，而（提取物名称2）表现出较低的抗氧化潜力。在200mg/kg体重剂量下，两种提取物的吞噬指数均显著增加，分别为5.2±0.2和4.8±0.1（p<0.001），这接近标准药物（左旋咪唑）100mg/kg体重时的5.4±0.2。两种提取物在200mg/kg体重剂量下也显著降低了扭体（腹部收缩）次数，分别为13.7±0.3和25.3±1.5（p<0.001），与标准药物双氯芬酸（双氯芬酸钠）相比，镇痛活性分别降低了71.8%和47.6%。总之，两种植物的提取物表明它们在管理各种疾病以缓解疼痛和调节免疫系统方面的作用。
