Department of Dermatology, University of Rochester, Rochester, New York 14627, United States.
Department of Biochemistry and Biophysics, University of Rochester, Rochester, New York 14627, United States.
ACS Sens. 2024 Apr 26;9(4):1799-1808. doi: 10.1021/acssensors.3c02225. Epub 2024 Mar 29.
Photonic technologies promise to deliver quantitative, multiplex, and inexpensive medical diagnostic platforms by leveraging the highly scalable processes developed for the fabrication of semiconductor microchips. However, in practice, the affordability of these platforms is limited by complex and expensive sample handling and optical alignment. We previously reported the development of a disposable photonic assay that incorporates inexpensive plastic micropillar microfluidic cards for sample delivery. That system as developed was limited to singleplex assays due to its optical configuration. To enable multiplexing, we report a new approach addressing multiplex light I/O, in which the outputs of individual grating couplers on a photonic chip are mapped to fibers in a fiber bundle. As demonstrated in the context of detecting antibody responses to influenza and SARS-CoV-2 antigens in human serum and saliva, this enables multiplexing in an inexpensive, disposable, and compact format.
光子学技术有望通过利用为制造半导体微芯片而开发的高度可扩展工艺，提供定量、多重和廉价的医疗诊断平台。然而，在实践中，这些平台的负担能力受到复杂和昂贵的样品处理和光学对准的限制。我们之前报道了一种可重复使用的光学生物传感器的开发，该传感器采用了廉价的塑料微柱微流控卡进行样品输送。由于其光学配置，该系统仅限于单重分析。为了实现多重检测，我们报告了一种新的多路光输入/输出方法，其中将光芯片上各个光栅耦合器的输出映射到光纤束中的光纤上。如在检测人血清和唾液中针对流感和 SARS-CoV-2 抗原的抗体反应的背景下所示，这使得在廉价、一次性和紧凑的格式中实现多重检测成为可能。
