Al-Jubori Sawsan Hameed, Al-Murad Maha A, Al-Mashhadane Faehaa Azher
Department of Conservative Dentistry, University of Mosul, Mosul, IRQ.
Department of Dental Basic Sciences, University of Mosul, Mosul, IRQ.
Cureus. 2022 May 26;14(5):e25360. doi: 10.7759/cureus.25360. eCollection 2022 May.
Aim Vitamin D3 plays an important role in affecting the overall remineralization process of the dentition. The use of supplements help to keep the levels at optimum and thus reduce the chances of treating very early lesion of caries. Hence the aim was to investigate the indirect effects of oral vitamin D3 on microhardness and elemental weight percentage of Calcium (Ca) and Phosphorous (P) in enamel surface with an artificially initiated carious lesion. Methods The 120 extracted premolars were randomly divided into five groups according to salivary immersion. Each group had a total of twenty-four participants, with the following characteristics: control +ve: sound enamel; control -ve: only subjected to pH cycle; A: pH cycle and immersion in control saliva; B: pH cycle and saliva collected after three weeks; and C: pH cycle and saliva collected after six weeks. The unstimulated saliva was collected from (40) adult volunteers receiving vitamin D3 1000IU gel capsules daily for six weeks. Before each vitamin D3 intake, 10 mL of unstimulated control saliva was collected from each participant. Then other 10 ml. were collected after three and six weeks of vitamin D receiving periods. Saliva immersion time (12 hours). Enamel surface was assessed by Vicker's Microhardness machine and (X-ray fluorescence - XRF) spectrometer. Results For all specimens, there was a significant decrease in both (Ca and P weight %) after demineralization and then they significantly increased after receiving vitamin D3. The microhardness and elemental analysis provide confirmed results that were represented as a statistically significant difference at (P≤ 0.05) between groups that received vitamin D3 and those without vitamin D3 dosage. Conclusions Oral vitamin D3 has a significant potential in motivating remineralization of early lesions on the enamel surfaces representing improved surface microhardness and minerals content (Ca and P weight %) of demineralized tooth surfaces.
维生素D3在影响牙列的整体再矿化过程中起着重要作用。补充剂的使用有助于将水平维持在最佳状态，从而减少治疗早期龋齿病变的几率。因此，本研究旨在探讨口服维生素D3对人工诱发龋损的牙釉质表面显微硬度以及钙（Ca）和磷（P）元素重量百分比的间接影响。方法：将120颗拔除的前磨牙根据唾液浸泡情况随机分为五组。每组共有24颗牙齿，具有以下特征：阳性对照组：健康牙釉质；阴性对照组：仅进行pH循环；A组：pH循环并浸泡于对照唾液中；B组：pH循环并浸泡于三周后收集的唾液中；C组：pH循环并浸泡于六周后收集的唾液中。从未刺激唾液中收集（40）名成年志愿者的唾液，这些志愿者每天服用维生素D3 1000IU凝胶胶囊，持续六周。在每次摄入维生素D3之前，从每位参与者收集10 mL未刺激的对照唾液。然后在接受维生素D的三周和六周后再收集10 mL。唾液浸泡时间为12小时。通过维氏显微硬度计和（X射线荧光 - XRF）光谱仪评估牙釉质表面。结果：对于所有标本，脱矿后钙和磷的重量百分比均显著降低，接受维生素D3后又显著增加。显微硬度和元素分析提供了确凿的结果，表明接受维生素D3的组与未接受维生素D3剂量的组之间在（P≤0.05）时有统计学显著差异。结论：口服维生素D3在促进牙釉质表面早期病变的再矿化方面具有显著潜力，表现为脱矿牙表面的表面显微硬度和矿物质含量（钙和磷的重量百分比）得到改善。
