Almoudi Manal Mohamed Mansour, Hussein Alaa Sabah, Abu Hassan Mohamed Ibrahim, Al-Talib Hassanain, Khan Hasnah Begum Said Gulam, Nazli Siti Arisya Binti, Effandy Nur Aina Efira Binti
Centre of Paediatric Dentistry and Orthodontics, Faculty of Dentistry, Universiti Teknologi MARA, Selangor, Malaysia.
Centre of Restorative Dentistry Studies, Faculty of Dentistry, Universiti Teknologi MARA, Selangor, Malaysia.
Eur Oral Res. 2021 Jan 4;55(1):8-15. doi: 10.26650/eor.20210119.
This study aims to evaluate the antimicrobial effects of the cholecalciferol vitamin D3 against Streptococcus sobrinus (Strep. sobrinus) and Streptococcus mutans (Strep. mutans) bacteria in vitro that is considered the main causative bacteria in dental caries development.
The antimicrobial effects of vitamin D3 were evaluated against Strep. sobrinus and Strep mutans using the agar disc diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of vitamin D3 were determined using a microdilution method following the guidelines by the Clinical Laboratory Standards Institute (CLSI). Scanning electron microscope (SEM) was used to evaluate the morphological changes of bacterial cells following exposure to vitamin D3.
Strep. sobrinus was more sensitive to vitamin D3 compared to Strep. mutans bacteria. The MIC values of vitamin D3 against Strep. sobrinus and Strep. mutans were 60 μg/ mL and 250 μg/mL respectively whereas the MBC values were 120 μg/mL and 500 μg/mL, respectively. Moreover, significant changes in the bacterial morphology were observed in treated bacterial cells with vitamin D3 as compared to the untreated control bacteria using SEM.
These findings suggested that vitamin D3 has excellent antimicrobial effects against Strep. sobrinus and Strep. mutans and may be considered as a promising compound in the prevention of dental caries in the future. Further research is recommended to elucidate the mechanism of vitamin D3 on these bacteria.
本研究旨在评估胆钙化醇维生素D3对变形链球菌和远缘链球菌的体外抗菌作用，这两种细菌被认为是龋齿发展的主要致病菌。
采用琼脂纸片扩散法评估维生素D3对远缘链球菌和变形链球菌的抗菌作用。按照临床实验室标准协会（CLSI）的指南，使用微量稀释法测定维生素D3的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。使用扫描电子显微镜（SEM）评估暴露于维生素D3后细菌细胞的形态变化。
与变形链球菌相比，远缘链球菌对维生素D3更敏感。维生素D3对远缘链球菌和变形链球菌的MIC值分别为60μg/mL和250μg/mL，而MBC值分别为120μg/mL和500μg/mL。此外，与未处理的对照细菌相比，使用SEM观察到用维生素D3处理的细菌细胞的形态有显著变化。
这些发现表明维生素D3对远缘链球菌和变形链球菌具有优异的抗菌作用，未来可能被视为预防龋齿的一种有前景的化合物。建议进一步研究以阐明维生素D3对这些细菌的作用机制。
