Department of Pathology, Hospital Universitário Antônio Pedro, Universidade Federal Fluminense, Niterói, RJ, Brazil.
Department of Dermatology, Hospital Universitário Antônio Pedro, Universidade Federal Fluminense, Niterói, RJ, Brazil.
An Bras Dermatol. 2024 Sep-Oct;99(5):688-695. doi: 10.1016/j.abd.2023.12.002. Epub 2024 Mar 29.
Merkel cell polyomavirus (MCPyV), a human polyomavirus that is unequivocally linked to merkel cell carcinoma (MCC), has been found in association with keratinocytes carcinomas (KC), especially basal cell carcinoma (BCC) and cutaneous squamous cell carcinoma (cSCC). Nevertheless, there is scarce information about the possible involvement of MCPyV in the development of KC.
To assess the presence of MCPyV DNA and Large-T Antigen (LT-Ag) via Polymerase Chain Reaction (PCR) and Immunohistochemistry (IHC) in cases of KC, and to correlate its presence with immunohistochemical markers p16, p53, and ki67, tumor type and subtype, sun-exposed location, and epidemiological data.
The prevalence of MCPyV DNA, LT-Ag, and immunohistochemical markers p16, p53, and ki67 was assessed by PCR and Immunohistochemistry (IHC) in 127 cases of KC, these results were correlated with tumor type and subtype, sun-exposed location, and epidemiological data.
The MCPyV DNA was detected in 42.57% (43 of 101) cases by PCR, the LT-Ag was detected in 16.4% (20 of 122) of cases, p16 in 81.5% (97 of 119), p53 in 66.4% (83 of 125), ki67 in 89% (73 of 82). No correlation between MCPyV LT-Ag and DNA confronted with tumor type, subtype, location site, and immunohistochemical markers was found. A single correlation between the MCPyV LT-Ag and cSCC tumors and peri-tumoral lymphocyte cells was noted.
Further steps need to be taken to better evaluate the MCPyV influence and its possible role in KC carcinogenesis, as the evaluation of the virus genome state, the gene sequence that encodes LT-Ag in the KC tumor cells, and in situ hybridization for viral DNA or RNA in these cells.
Despite the frequent detection of MCPyV in KC, the data available so far does not support the hypothesis of a causal relationship between them.
默克尔细胞多瘤病毒（MCPyV）是一种与人相关的多瘤病毒，与默克尔细胞癌（MCC）有明确联系，已被发现与角质形成细胞癌（KC）有关，特别是基底细胞癌（BCC）和皮肤鳞状细胞癌（cSCC）。然而，关于 MCPyV 可能参与 KC 发展的信息很少。
通过聚合酶链反应（PCR）和免疫组织化学（IHC）评估 KC 病例中 MCPyV DNA 和大 T 抗原（LT-Ag）的存在，并将其与免疫组织化学标志物 p16、p53 和 ki67、肿瘤类型和亚型、暴露于阳光的位置以及流行病学数据相关联。
通过 PCR 和免疫组织化学（IHC）评估了 127 例 KC 中 MCPyV DNA、LT-Ag 和免疫组织化学标志物 p16、p53 和 ki67 的流行率，这些结果与肿瘤类型和亚型、暴露于阳光的位置和流行病学数据相关联。
PCR 检测到 42.57%（43/101）的病例存在 MCPyV DNA，16.4%（20/122）的病例存在 LT-Ag，81.5%（97/119）的病例存在 p16，66.4%（83/125）的病例存在 p53，89%（73/82）的病例存在 ki67。未发现 MCPyV LT-Ag 与肿瘤类型、亚型、位置和免疫组织化学标志物之间存在相关性。仅发现 MCPyV LT-Ag 与 cSCC 肿瘤和肿瘤周围淋巴细胞细胞之间存在单一相关性。
需要采取进一步措施来更好地评估 MCPyV 的影响及其在 KC 癌变中的可能作用，如评估病毒基因组状态、KC 肿瘤细胞中编码 LT-Ag 的基因序列以及这些细胞中的病毒 DNA 或 RNA 的原位杂交。
尽管在 KC 中经常检测到 MCPyV，但到目前为止的数据并不支持它们之间存在因果关系的假设。
