Department of Pulmonary and Critical Care Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Institute of Respiratory Diseases, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Brief Bioinform. 2024 Mar 27;25(3). doi: 10.1093/bib/bbae124.
Coronavirus disease 2019 (COVID-19) has been wreaking havoc for 3 years. PANoptosis, a distinct and physiologically relevant inflammatory programmed cell death, perpetuates cytokine storm and multi-organ injuries in COVID-19. Although PANoptosis performs indispensable roles in host defense, further investigation is needed to elucidate the exact processes through which PANoptosis modulates immunological responses and prognosis in COVID-19. This study conducted a bioinformatics analysis of online single-cell RNA sequence (scRNA-seq) and bulk RNA-seq datasets to explore the potential of PANoptosis as an indicator of COVID-19 severity. The degree of PANoptosis in bronchoalveolar lavage fluid (BALF) and peripheral blood mononuclear cells (PBMC) indicated the severity of COVID-19. Single-cell transcriptomics identified pro-inflammatory monocytes as one of the primary sites of PANoptosis in COVID-19. The study subsequently demonstrated the immune and metabolic characteristics of this group of pro-inflammatory monocytes. In addition, the analysis illustrated that dexamethasone was likely to alleviate inflammation in COVID-19 by mitigating PANoptosis. Finally, the study showed that the PANoptosis-related genes could predict the intensive care unit admission (ICU) and outcomes of COVID-19 patients who are hospitalized.
新型冠状病毒病 2019（COVID-19）已经肆虐了 3 年。PANoptosis，一种独特的、与生理相关的炎症程序性细胞死亡，会导致 COVID-19 中的细胞因子风暴和多器官损伤。尽管 PANoptosis 在宿主防御中发挥着不可或缺的作用，但仍需要进一步研究来阐明 PANoptosis 调节 COVID-19 免疫反应和预后的确切过程。本研究对在线单细胞 RNA 测序（scRNA-seq）和批量 RNA-seq 数据集进行了生物信息学分析，以探讨 PANoptosis 作为 COVID-19 严重程度指标的潜力。支气管肺泡灌洗液（BALF）和外周血单核细胞（PBMC）中 PANoptosis 的程度表明 COVID-19 的严重程度。单细胞转录组学鉴定出促炎单核细胞是 COVID-19 中 PANoptosis 的主要发生部位之一。随后，该研究展示了这群促炎单核细胞的免疫和代谢特征。此外，分析表明地塞米松可能通过减轻 PANoptosis 来减轻 COVID-19 的炎症。最后，该研究表明，PANoptosis 相关基因可以预测住院 COVID-19 患者入住重症监护病房（ICU）和结局。
