Wada S, Ozaki Y, Nishio S, Maekawa M, Kimura S, Kinoshita Y
J Natl Cancer Inst. 1985 Mar;74(3):659-64.
Bladder tumors were induced in Wistar rats by oral administration of N-butyl-N-(4-hydroxybutyl)nitrosamine [(BBN) CAS: 3817-11-6], after which changes in bladder mucous membranes and in the response of thymus lymphocytes to concanavalin A (Con A) were studied. The response of thymus lymphocytes to Con A began to decrease from the 8th week after BBN administration, with changes such as hypertrophy or vascular formation in the bladder membrane. The response decreased markedly from the 15th week, when the tumors became visible. At about the 20th week, approximately 95% of the rats had bladder tumors. When thymus lymphocytes were pretreated with thymosin fraction 5 extracted from calf thymus, the response to Con A improved between the 8th and 20th weeks. When the thymus small-lymphocyte population was separated into lighter, intermediate, and heavier subpopulations by discontinuous density gradient centrifugation and an adhesion column, the effect of thymosin fraction 5 was much greater in the lighter subpopulation.
通过给Wistar大鼠口服N-丁基-N-(4-羟丁基)亚硝胺[(BBN)，CAS: 3817-11-6]诱导膀胱肿瘤，之后研究膀胱黏膜的变化以及胸腺淋巴细胞对刀豆球蛋白A(Con A)的反应。胸腺淋巴细胞对Con A的反应在给予BBN后的第8周开始下降，同时膀胱黏膜出现肥大或血管形成等变化。从第15周肿瘤可见时起，反应明显下降。在大约第20周时，约95%的大鼠患有膀胱肿瘤。当用从小牛胸腺中提取的胸腺素组分5预处理胸腺淋巴细胞时，在第8周至第20周期间对Con A的反应有所改善。当通过不连续密度梯度离心和黏附柱将胸腺小淋巴细胞群体分离为较轻、中等和较重的亚群时，胸腺素组分5在较轻亚群中的作用要大得多。
