体内溶剂抑制局部氢核磁共振波谱法：通往代谢的窗口？

Bottomley P A, Edelstein W A, Foster T H, Adams W A

Proc Natl Acad Sci U S A. 1985 Apr;82(7):2148-52. doi: 10.1073/pnas.82.7.2148.

Solvent-suppression NMR techniques are combined with a pulsed magnetic field gradient and surface coil detection method of spatial localization. The result is a technique that enables observation of metabolites in the hydrogen (1H) NMR chemical-shift spectra from preselected disk-shaped volumes of biological tissue in vivo. Localized spectra are recorded from the normal human brain and forearm and from a dog in acquisition periods of 2 s using a 1.5-T imaging/spectroscopy system. This is several hundred-fold faster than acquiring similar state-of-the-art 31P NMR spectra of brain metabolites in vivo. Spectroscopy experiments are followed by conventional surface coil imaging sequences to precisely define the selected volume. Contamination of spectra by lipid resonances is a problem.

溶剂抑制核磁共振技术与脉冲磁场梯度和表面线圈空间定位检测方法相结合。结果得到一种技术，能够在体内从生物组织的预选盘状体积中观察氢（1H）核磁共振化学位移谱中的代谢物。使用1.5-T成像/光谱系统，在2秒的采集期内从正常人类大脑、前臂以及一只狗身上记录局部光谱。这比在体内获取类似的脑代谢物的最新31P核磁共振谱快几百倍。光谱实验之后是传统的表面线圈成像序列，以精确界定所选体积。脂质共振对光谱的污染是一个问题。