利用合成肽抗原高效生成针对癌蛋白的抗体。
Efficient generation of antibodies to oncoproteins by using synthetic peptide antigens.
作者信息
Tanaka T, Slamon D J, Cline M J
出版信息
Proc Natl Acad Sci U S A. 1985 May;82(10):3400-4. doi: 10.1073/pnas.82.10.3400.
Abstract
To examine the efficiency of generating protein-reactive antipeptide antibodies, 35 peptides encoded by retroviral or cellular oncogenes were used to immunize rabbits. Thirty-two peptides elicited antipeptide antibodies, of which 56% reacted with their respective oncoproteins. The length of the immunizing peptide was an important factor in generating antibodies reactive with native protein. Similar peptides differing in a single or a few amino acids could elicite antisera of markedly different reactivities.
摘要
为检测产生蛋白质反应性抗肽抗体的效率，使用35种由逆转录病毒或细胞癌基因编码的肽免疫兔子。32种肽引发了抗肽抗体，其中56%与各自的癌蛋白发生反应。免疫肽的长度是产生与天然蛋白反应的抗体的一个重要因素。单个或少数氨基酸不同的相似肽可引发反应性明显不同的抗血清。
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