Boyle W J, Lipsick J S, Reddy E P, Baluda M A
Proc Natl Acad Sci U S A. 1983 May;80(10):2834-8. doi: 10.1073/pnas.80.10.2834.
The genome of the replication-defective avian myeloblastosis virus (AMV) contains an inserted cellular sequence (amv) that is part of the oncogene responsible for acute myeloblastic leukemia in chickens infected with AMV. Three antisera raised against distinct synthetic peptides predicted from the long open reading frame of amv specifically precipitated the same 48-kilodalton protein (p48amv) from leukemic myeloblasts but not from normal hematopoietic tissue, fibroblasts, or from fibroblasts infected with the AMV helper virus, MAV-1 (myeloblastosis-associated virus type 1). p48amv is not glycosylated or phosphorylated and does not appear to act as a protein kinase in vitro. The same three antisera that recognized p48amv also specifically precipitated a common 110-kilodalton protein from normal uninfected hematopoietic tissue. This normal cellular homologue of the AMV leukemogenic protein, p110proto-amv, was not present in normal fibroblasts, MAV-1 infected fibroblasts, or, interestingly, in some leukemic myeloblasts. We conclude that p48amv is the leukemogenic product of an altered, transduced, partial protooncogene. Short helper-virus sequences provide its carboxyl terminus and also may provide the amino terminus.
复制缺陷型禽成髓细胞瘤病毒（AMV）的基因组包含一个插入的细胞序列（amv），该序列是导致感染AMV的鸡发生急性髓细胞白血病的癌基因的一部分。针对从amv的长开放阅读框预测的不同合成肽产生的三种抗血清，特异性地从白血病成髓细胞中沉淀出相同的48千道尔顿蛋白（p48amv），但未从正常造血组织、成纤维细胞或感染AMV辅助病毒MAV-1（1型成髓细胞瘤相关病毒）的成纤维细胞中沉淀出该蛋白。p48amv未进行糖基化或磷酸化修饰，在体外似乎也不具有蛋白激酶的作用。识别p48amv的相同三种抗血清还从正常未感染的造血组织中特异性沉淀出一种常见的110千道尔顿蛋白。这种AMV致白血病蛋白的正常细胞同源物p110proto-amv，在正常成纤维细胞、感染MAV-1的成纤维细胞中不存在，有趣的是，在一些白血病成髓细胞中也不存在。我们得出结论，p48amv是一个改变的、转导的部分原癌基因的致白血病产物。短的辅助病毒序列提供其羧基末端，也可能提供氨基末端。
