Tanaka T, Ida N, Shimoda H, Waki C, Slamon D J, Cline M J
Mol Cell Biochem. 1986 Apr;70(1):97-104. doi: 10.1007/BF00233807.
Expression of proteins encoded by the ras proto-oncogenes was examined in extracts from normal rat organs using anti-ras p21 antibodies generated against synthetic peptides. The highest level of ras p21 was found in brain (cerebrum) and was predominantly of c-Ha-ras origin. Levels of brain ras p21 did not vary from the newborn period of 3 months of age. Moderate levels of ras p21s were detected in lung, spleen and thymus. In contrast to the p21 in brain, these levels varied with the age of the rats and were encoded by other members of ras proto-oncogene family (Ki-ras or N-ras). This organ specific expression of different ras genes might be related to developmental control of gene expressions.
利用针对合成肽产生的抗ras p21抗体，在正常大鼠器官提取物中检测了ras原癌基因编码的蛋白质表达。发现脑（大脑）中ras p21水平最高，且主要来源于c-Ha-ras。脑ras p21水平在出生至3个月龄期间没有变化。在肺、脾和胸腺中检测到中等水平的ras p21。与脑中的p21不同，这些水平随大鼠年龄而变化，并且由ras原癌基因家族的其他成员（Ki-ras或N-ras）编码。不同ras基因的这种器官特异性表达可能与基因表达的发育控制有关。
