Dutta Saurav Kumar, Singh Chandra Vijay, Hans Manoj Kumar, Bathla Saurav, Singh Varnika, Mishra Soumya
Post Graduate Student, Department of Conservative Dentistry and Endodontics, Institute of Dental Sciences, Bareilly, Uttar Pradesh, India.
Professor, Department of Conservative Dentistry and Endodontics, Institute of Dental Sciences, Bareilly, Uttar Pradesh, India.
J Pharm Bioallied Sci. 2024 Feb;16(Suppl 1):S619-S622. doi: 10.4103/jpbs.jpbs_901_23. Epub 2024 Feb 29.
The aim and objective of this study was to evaluate the antibacterial efficacy of mineral trioxide aggregate, bioactive glass sealer, and epoxy-resin-based sealer.
In the present study, 22 Mueller Hinton agar (MH agar) plates were employed and equally divided into two groups. Three holes were made by removal of agar at equidistant points and filled with root canal sealers. The strains of the bacteria used in this study were (ATCC 6538) and (ATCC 10231) and were divided into two groups and root canal sealers were divided into three subgroups: mineral trioxide aggregate (MTA) fillapex Sealer, Nishika Bioactive Glass sealer, and Syntex Epoxy Resin base sealer. For , peptone water was placed in a 2 mL test tube and bacteria were extracted from blood agar plates using a nichrome wire loop and poured into the peptone water-containing test tube and incubated for 2 hours and for , fungi were grown at 37°C for 24 hours in MH Broth and seeded into MH agar to produce turbidity of 0.5 on the McFarland scale, which corresponds to a concentration of 10 CFU/mL. This MH broth was used as a second layer. The seeded agar was then added over the plates immediately after the insertion of sealer cement. After incubation, the diameters of zones of inhibition around the plates were measured.
The results of this study showed that the highest inhibition was recorded in Syntex sealer against followed by MTA fillapex sealer and Nishika sealer, whereas MTA fillapex showed the highest inhibition against followed by Syntex sealer and Nishika sealer.
Syntex sealer exhibits better antibacterial efficacy against and MTA fillapex exhibit better antibacterial efficacy against .
本研究的目的是评估三氧化矿物凝聚体、生物活性玻璃封闭剂和环氧树脂基封闭剂的抗菌效果。
在本研究中，使用了22个穆勒-欣顿琼脂（MH琼脂）平板，并将其平均分为两组。通过在等距点去除琼脂制作三个孔，并用根管封闭剂填充。本研究中使用的细菌菌株为（ATCC 6538）和（ATCC 10231），并分为两组，根管封闭剂分为三个亚组：三氧化矿物凝聚体（MTA）Fillapex封闭剂、Nishika生物活性玻璃封闭剂和Syntex环氧树脂基封闭剂。对于，将蛋白胨水置于2 mL试管中，使用镍铬合金丝环从血琼脂平板中提取细菌并倒入含蛋白胨水的试管中，孵育2小时，对于，真菌在MH肉汤中于37°C培养24小时，然后接种到MH琼脂中，使其在麦氏比浊管上产生0.5的浊度，这相当于浓度为10 CFU/mL。该MH肉汤用作第二层。在插入封闭剂水泥后，立即将接种的琼脂添加到平板上。孵育后，测量平板周围抑菌圈的直径。
本研究结果表明，Syntex封闭剂对的抑菌效果最高，其次是MTA Fillapex封闭剂和Nishika封闭剂，而MTA Fillapex对的抑菌效果最高，其次是Syntex封闭剂和Nishika封闭剂。
Syntex封闭剂对表现出更好的抗菌效果，而MTA Fillapex对表现出更好的抗菌效果。
