Engineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou, 730050, China.
Cell Biology and Immunology Group, Wageningen University & Research, Wageningen, The Netherlands.
Sci Rep. 2024 Apr 10;14(1):8413. doi: 10.1038/s41598-024-58933-8.
Strain-specific probiotics can present antioxidant activity and reduce damage caused by oxidation. Streptococcus alactolyticus strain FGM (S. alactolyticus strain FGM) isolated from the chicken cecum shows potential probiotic properties which have been previously demonstrated. However, the antioxidant properties of S. alactolyticus strain FGM remain unknown. In this view, cell-free supernatant (CFS), intact cells (IC) and intracellular extracts (CFE) of strain FGM and 3 strains of Lactobacillus (LAB) were prepared, and their scavenging capacities against DPPH, hydroxyl radicals and linoleic acid peroxidation inhibitory were compared in this study. The effects of strain FGM cell-free supernatant (FCFS) on NO production, activity of SOD and GSH-Px in RAW264.7 cells and LPS-induced RAW264.7 cells were analyzed. The metabolites in the supernatant were quantitated by N300 Quantitative Metabolome. It was shown that the physicochemical characteristics of CFS to scavenge DPPH, hydroxyl radicals, and linoleic acid peroxidation inhibitory were significantly stronger than that of IC and CFE in the strain FGM (P < 0.05), respectively 87.12% ± 1.62, 45.03% ± 1.27, 15.63% ± 1.34. FCFS had a promotional effect on RAW264.7 cells, and significantly elevated SOD and GSH-Px activities in RAW264.7 cells. 25 μL FCFS significantly promoted the proliferation of RAW264.7 cells induced by LPS, increased the activities of SOD and GSH-PX, and decreased the release of NO. Furthermore, among the differential metabolites of FCFS quantified by N300, 12 metabolites were significantly up-regulated, including lactic acid, indole lactic acid, linoleic acid, pyruvic acid etc., many of which are known with antioxidant properties. In conclusion, FCFS had good antioxidant properties and activity, which can be attributed to metabolites produced from strain FGM fermentation. It was further confirmed that S. alactolyticus strain FGM and its postbiotic have potential probiotic properties and bright application prospects in livestock and poultry breeding.
从鸡盲肠中分离出的嗜热链球菌 FGM（Streptococcus alactolyticus strain FGM）具有潜在的益生菌特性，此前已经证明了这一点。然而，嗜热链球菌 FGM 的抗氧化特性尚不清楚。在本研究中，制备了 FGM 菌株和 3 株乳杆菌（Lactobacillus，LAB）的无细胞上清液（cell-free supernatant，CFS）、完整细胞（intact cells，IC）和细胞内提取物（intracellular extracts，CFE），并比较了它们清除 DPPH、羟基自由基和亚油酸过氧化抑制的能力。分析了 FGM 无细胞上清液（FCFS）对 RAW264.7 细胞中 NO 产生、SOD 和 GSH-Px 活性的影响以及 LPS 诱导的 RAW264.7 细胞。通过 N300 定量代谢组学对上清液中的代谢物进行定量分析。结果表明，CFS 的理化特性在清除 DPPH、羟基自由基和亚油酸过氧化抑制方面明显强于 FGM 菌株的 IC 和 CFE（P < 0.05），分别为 87.12%±1.62、45.03%±1.27 和 15.63%±1.34。FCFS 对 RAW264.7 细胞具有促进作用，显著提高了 RAW264.7 细胞中 SOD 和 GSH-Px 的活性。25 μL FCFS 可显著促进 LPS 诱导的 RAW264.7 细胞增殖，提高 SOD 和 GSH-PX 的活性，降低 NO 的释放。此外，通过 N300 定量的 FCFS 差异代谢物中，有 12 种代谢物显著上调，包括乳酸、吲哚乳酸、亚油酸、丙酮酸等，其中许多都具有抗氧化特性。综上所述，FCFS 具有良好的抗氧化活性，这归因于 FGM 菌株发酵产生的代谢物。进一步证实，嗜热链球菌 FGM 及其后生元具有潜在的益生菌特性，在畜牧业和家禽养殖中有广阔的应用前景。
