Altieri Clelia, Filippone Alfonso, Bevilacqua Antonio, Corbo Maria Rosaria, Sinigaglia Milena
Department of Agriculture, Food, Natural Resources and Engineering, University of Foggia, 71122 Foggia, Italy.
Department of Psychology and Education, Pegaso University, 80143 Napoli, Italy.
Foods. 2024 Sep 19;13(18):2966. doi: 10.3390/foods13182966.
Three strains of and three bifidobacteria ( subsp. , , and ) were used as target strains; in addition, for each microorganism, the cell-free supernatant (CFS) was produced and used as an ingredient of the growth medium. Namely CFSs from lactobacilli were used on bifidobacteria and CFSs from bifidobacteria were used on lactobacilli. The viable count was assessed, and the data were modelled through a reparametrized Gompertz equation cast both in the positive and negative form to evaluate the parameters t-7log, which is the time after which the viable count was 7 log CFU/mL, and the t-7log*, which is the time after which the viable count was below 7 log CFU/mL; the difference between the t-7log* and t-7log defines the stability time. Statistics through a multiparametric ANOVA (analysis of variance) provided evidence for the presence of a bifidogenic and/or bioactive factor produced by bifidobacteria and active on lactobacilli, and vice versa (bioactive factor of lactobacilli with a functional effect on bifidobacteria), although further studies are required to better explain the mechanisms beyond the positive effects. In addition, the influence on the target strains can be found during the growth phase (stimulation), as well as during senescence and death phase (protective effect), with a strong strain/species dependence on both CFS production and target strain.
使用了三株[乳酸菌属]菌株和三株双歧杆菌（[双歧杆菌亚种]、[双歧杆菌亚种]和[双歧杆菌亚种]）作为目标菌株；此外，对于每种微生物，制备了无细胞上清液（CFS）并将其用作生长培养基的一种成分。即来自乳酸菌的CFS用于双歧杆菌，来自双歧杆菌的CFS用于乳酸菌。评估活菌计数，并通过以正、负形式转换的重新参数化Gompertz方程对数据进行建模，以评估参数t-7log（即活菌计数达到7 log CFU/mL后的时间）和t-7log*（即活菌计数低于7 log CFU/mL后的时间）；t-7log*与t-7log之间的差值定义了稳定时间。通过多参数方差分析（ANOVA）进行的统计为双歧杆菌产生并对乳酸菌有活性的双歧因子和/或生物活性因子的存在提供了证据，反之亦然（乳酸菌的生物活性因子对双歧杆菌有功能作用），尽管需要进一步研究以更好地解释这些积极作用背后的机制。此外，在生长阶段（刺激）以及衰老和死亡阶段（保护作用）都能发现对目标菌株的影响，且对CFS产生和目标菌株都有很强的菌株/物种依赖性。
