Bratke Sebastian, Schmid Sebastian, Ulm Bernhard, Jungwirth Bettina, Blobner Manfred, Borgstedt Laura
Department of Anesthesiology and Intensive Care Medicine, Faculty of Medicine, University of Ulm, Ulm, Germany.
Department of Anesthesiology and Intensive Care Medicine, School of Medicine, Technical University of Munich, Munich, Germany.
Front Med (Lausanne). 2024 Mar 27;11:1342752. doi: 10.3389/fmed.2024.1342752. eCollection 2024.
The prevalence of neurodegenerative diseases is increasing as is life expectancy with Alzheimer's disease accounting for two-thirds of dementia cases globally. Whether general anesthesia and surgery worsen cognitive decline is still a matter of debate and most likely depending on the interplay of various influencing factors. In order to account for this complexity, Alzheimer's disease animal models have been developed. The Tg2576 model of Alzheimer's disease is a well-established mouse model exhibiting amyloidopathy and age-dependent sex-specific differences in Alzheimer's disease symptomology. Yet, data on anesthesia in this mouse model is scarce and a systematic comparison of vital parameters during anesthesia with wild-type animals is missing. In order to investigate the safety of general anesthesia and changes in vital parameters during general anesthesia in Tg2576 mice, we did a secondary analysis of vital parameters collected during general anesthesia in aged Tg2576 mice.
After governmental approval (General Administration of the Free State of Bavaria, file number: 55.2-1-54-2532-149-11) 60 mice at 10-12 months of age were exposed to isoflurane (1.6 Vol%) for 120 min, data of 58 mice was analyzed. During general anesthesia, heart rate, respiratory rate, temperature, isoflurane concentration and fraction of inspired oxygen were monitored and collected. Data were analyzed using univariate and multivariate linear mixed regression models.
During general anesthesia, heart rate decreased in a sex-specific manner. Respiratory rate decreased and body temperature increased dependent on genotype. However, the changes were limited and all vital parameters stayed within physiological limits.
Isoflurane anesthesia in the Tg2576 mouse model is safe and does not seem to influence experimental results by interacting with vital parameters. The present study provides information on appropriate anesthesia in order to advance research on anesthesia and AD and could contribute to improving laboratory animal welfare.
随着预期寿命的增加，神经退行性疾病的患病率也在上升，其中阿尔茨海默病占全球痴呆病例的三分之二。全身麻醉和手术是否会加剧认知衰退仍是一个有争议的问题，很可能取决于各种影响因素的相互作用。为了应对这种复杂性，已经开发了阿尔茨海默病动物模型。阿尔茨海默病的Tg2576模型是一种成熟的小鼠模型，表现出淀粉样病变以及阿尔茨海默病症状学中与年龄相关的性别特异性差异。然而，关于这种小鼠模型麻醉的数据很少，并且缺少与野生型动物在麻醉期间生命体征参数的系统比较。为了研究Tg2576小鼠全身麻醉的安全性以及全身麻醉期间生命体征参数的变化，我们对老年Tg2576小鼠全身麻醉期间收集的生命体征参数进行了二次分析。
经政府批准（巴伐利亚自由州总管理局，文件编号：55.2 - 1 - 54 - 2532 - 149 - 11），60只10 - 12月龄的小鼠暴露于异氟烷（1.6体积%）120分钟，分析了58只小鼠的数据。在全身麻醉期间，监测并收集心率、呼吸频率、体温、异氟烷浓度和吸入氧分数。使用单变量和多变量线性混合回归模型分析数据。
在全身麻醉期间，心率以性别特异性方式下降。呼吸频率下降，体温升高，这取决于基因型。然而，这些变化是有限的，所有生命体征参数都保持在生理范围内。
Tg2576小鼠模型中的异氟烷麻醉是安全的，似乎不会通过与生命体征参数相互作用而影响实验结果。本研究提供了关于适当麻醉的信息，以推进麻醉与阿尔茨海默病的研究，并有助于改善实验动物福利。
