Key Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People's Republic of China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
Antonie Van Leeuwenhoek. 2021 Jun;114(6):697-708. doi: 10.1007/s10482-021-01550-6. Epub 2021 Mar 5.
A novel actinobacterium, designated strain NEAU-HG-1, was isolated from soil collected from Harbin, Heilongjiang Province, Northeast China and characterised using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain NEAU-HG-1 belonged to the genus Micromonospora, and shared high sequence similarities with Micromonospora auratinigra DSM 44815 (98.9%) and Micromonospora coerulea DSM 43143 (98.7%). Morphological and chemotaxonomic characteristics of the strain also supported its assignment to the genus Micromonospora. Cell wall contained meso-diaminopimelic acid and the whole-cell sugars were arabinose and xylose. The polar lipid contained diphosphatidylglycerol, phosphatidylethanolamine, glycolipid and phosphatidylinositol. The predominant menaquinones were MK-10(H), MK-10(H) and MK-10(H). The major fatty acids were C cycle, iso-C, and iso-C. Furthermore, strain NEAU-HG-1 displayed a DNA-DNA relatedness of 33.8 ± 2.2% with M. coerulea DSM 43143. The level of digital DNA-DNA hybridization between strain NEAU-HG-1 and M. auratinigra DSM 44815 was 27.2% (24.8-29.7%). The value was well below the criteria for species delineation of 70% for dDDH. Whole-genome average nucleotide identity analyses result also indicated that the isolate should be assigned to a new species under the genus Micromonospora. Therefore, it is concluded that strain NEAU-HG-1 represents a novel species of the genus Micromonospora, for which the name Micromonospora rubida sp. nov. is proposed, with NEAU-HG-1 (= CGMCC 4.7479 = JCM 32386) as the type strain.
一株新的放线菌，命名为 NEAU-HG-1 菌株，从中国东北黑龙江省哈尔滨采集的土壤中分离得到，并通过多相方法进行了特征描述。基于 16S rRNA 基因序列分析，菌株 NEAU-HG-1 属于 Micromonospora 属，与 Micromonospora auratinigra DSM 44815（98.9%）和 Micromonospora coerulea DSM 43143（98.7%）具有很高的序列相似性。该菌株的形态学和化学分类学特征也支持其归属于 Micromonospora 属。细胞壁含有 meso-二氨基庚二酸，全细胞糖为阿拉伯糖和木糖。极性脂含有双磷脂酰甘油、磷脂酰乙醇胺、糖脂和磷脂酰肌醇。主要的类异戊二烯醌为 MK-10(H)、MK-10(H)和 MK-10(H)。主要脂肪酸为 C 环、iso-C 和 iso-C。此外，菌株 NEAU-HG-1 与 M. coerulea DSM 43143 的 DNA-DNA 同源性为 33.8±2.2%。菌株 NEAU-HG-1 与 M. auratinigra DSM 44815 的数字 DNA-DNA 杂交水平为 27.2%（24.8-29.7%）。该值远低于种间界定的 70%的 dDDH 标准。全基因组平均核苷酸同一性分析结果也表明，该分离株应属于 Micromonospora 属的一个新种。因此，结论是菌株 NEAU-HG-1 代表了 Micromonospora 属的一个新种，建议命名为 Micromonospora rubida sp. nov.，其模式菌株为 NEAU-HG-1（=CGMCC 4.7479=JCM 32386）。
