Wang Kaitong, Zhang Ning, Fu Xue, Zhang Huanhuan, Liu Shengyan, Pu Xue, Wang Xiao, Si Huaijun
State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, China.
College of Agronomy, Gansu Agricultural University, Lanzhou, China.
Front Plant Sci. 2022 Sep 16;13:1009552. doi: 10.3389/fpls.2022.1009552. eCollection 2022.
The major stages of the potato life cycle are tuber dormancy and sprouting, however, there is still known very little of the mechanisms that control these processes. TCP (Theosinte branch I, Cycloidea, proliferationcell factors 1 and 2) transcription factors play a key role in plant growth and dormancy related developmental processes. Previous researches demonstrated that TCP transcription factor had a function in the promotion of dormancy. To elucidate the function of gene, it was cloned from potato cultivar "Desiree," which encodes a polypeptide consisting of 414 amino acids and is mainly found in the nucleus. The potato tubers of overexpression lines sprouted in advance, while the potato tubers of down-regulated expression lines showed delayed sprouting. In addition, it was also found that overexpression lines of extremely significantly reduced the ratio of abscisic acid (ABA)/gibberellic acid (GA), while the superoxide dismutase activity decreased, and the activity of peroxidase and catalase increased compared with the wild type. The opposite result was found in the down-regulated expression lines of gene. Three interacting proteins, StSnRK1, StF-Box and StGID1, were screened by Yeast two-hybrid, and verified by Bimolecular Fluorescence Complementation and Split-luciferase, indicating that could affect ABA and GA signaling pathways to regulate potato tuber dormancy and sprouting. Together, these results demonstrated that regulated potato tuber dormancy and sprouting by affecting the dynamic balance between ABA and GA. The result could provide some information on the molecular mechanism of regulating potato tuber dormancy and sprouting.
马铃薯生命周期的主要阶段是块茎休眠和发芽，然而，目前对控制这些过程的机制仍知之甚少。TCP（玉米分枝Ⅰ、Cycloidea、增殖细胞因子1和2）转录因子在植物生长和与休眠相关的发育过程中起关键作用。先前的研究表明，TCP转录因子具有促进休眠的功能。为了阐明该基因的功能，从马铃薯品种“德西蕾”中克隆了该基因，它编码一个由414个氨基酸组成的多肽，主要存在于细胞核中。该基因过表达系的马铃薯块茎提前发芽，而下调表达系的马铃薯块茎发芽延迟。此外，还发现该基因过表达系的脱落酸（ABA）/赤霉素（GA）比值极显著降低，而超氧化物歧化酶活性降低，过氧化物酶和过氧化氢酶活性相对于野生型升高。在该基因的下调表达系中发现了相反的结果。通过酵母双杂交筛选出三个相互作用蛋白，即StSnRK1、StF-Box和StGID1，并通过双分子荧光互补和分裂荧光素酶进行了验证，表明该基因可以影响ABA和GA信号通路来调节马铃薯块茎的休眠和发芽。总之，这些结果表明该基因通过影响ABA和GA之间的动态平衡来调节马铃薯块茎的休眠和发芽。该结果可为该基因调控马铃薯块茎休眠和发芽的分子机制提供一些信息。
