College of Life Science, Northeast Forestry University, Harbin 150040, China.
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China.
Int J Mol Sci. 2024 Apr 8;25(7):4116. doi: 10.3390/ijms25074116.
Ethylene (ET) is an important phytohormone that regulates plant growth, development and stress responses. The ethylene-insensitive3/ethylene-insensitive3-like (EIN3/EIL) transcription factor family, as a key regulator of the ET signal transduction pathway, plays an important role in regulating the expression of ET-responsive genes. Although studies of EIN3/EIL family members have been completed in many species, their role in doubled haploid (DH) poplar derived from another culture of diploid × (donor tree, DT) remains ambiguous. In this study, a total of seven EIN3/EIL gene family members in the DH poplar genome were identified. Basic physical and chemical property analyses of these genes were performed, and these proteins were predicted to be localized to the nucleus. According to the phylogenetic relationship, EIN3/EIL genes were divided into two groups, and the genes in the same group had a similar gene structure and conserved motifs. The expression patterns of EIN3/EIL genes in the apical buds of different DH poplar plants were analyzed based on transcriptome data. At the same time, the expression patterns of , , and genes in different tissues of different DH plants were detected via RT-qPCR, including the apical buds, young leaves, functional leaves, xylem, cambium and roots. The findings presented above indicate notable variations in the expression levels of genes across various tissues of distinct DH plants. Finally, the gene was overexpressed in DT, and the transgenic plants showed a dwarf phenotype, indicating that the gene was involved in regulating the growth and development of poplar. In this study, the EIN3/EIL gene family of DH poplar was analyzed and functionally characterized, which provides a theoretical basis for the future exploration of the EIN3/EIL gene function.
乙烯（ET）是一种重要的植物激素，调节植物的生长、发育和应激反应。乙烯不敏感 3/乙烯不敏感 3 样（EIN3/EIL）转录因子家族作为 ET 信号转导途径的关键调节因子，在调节 ET 响应基因的表达方面发挥着重要作用。尽管在许多物种中已经完成了 EIN3/EIL 家族成员的研究，但它们在来自另一种二倍体 × （供体树，DT）培养物的 DH 杨树上的作用仍不清楚。在这项研究中，共鉴定出 DH 杨树基因组中的七个 EIN3/EIL 基因家族成员。对这些基因进行了基本的理化性质分析，并预测这些蛋白质定位于细胞核。根据系统发育关系，EIN3/EIL 基因分为两组，同一组中的基因具有相似的基因结构和保守基序。根据转录组数据，分析了不同 DH 杨树植株顶芽中 EIN3/EIL 基因的表达模式。同时，通过 RT-qPCR 检测了不同 DH 植物不同组织中 、 、 和 基因的表达模式，包括顶芽、幼叶、功能叶、木质部、形成层和根。上述结果表明，不同 DH 植物不同组织中 基因的表达水平存在显著差异。最后，在 DT 中转基因过表达 基因，转基因植株表现出矮化表型，表明 基因参与调控杨树的生长发育。本研究对 DH 杨树的 EIN3/EIL 基因家族进行了分析和功能表征，为今后探索 EIN3/EIL 基因功能提供了理论依据。
