Alinaghi Somayeh, Mohseni Marzieh, Fattahi Zohreh, Beheshtian Maryam, Ghodratpour Fatemeh, Zare Ashrafi Farzane, Arzhangi Sanaz, Jalalvand Khadijeh, Najafipour Reza, Khorram Khorshid Hamid Reza, Kahrizi Kimia, Najmabadi Hossein
Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.
Arch Iran Med. 2024 Feb 1;27(2):79-88. doi: 10.34172/aim.2024.13.
The study of Y-chromosomal variations provides valuable insights into male susceptibility in certain diseases like cardiovascular disease (CVD). In this study, we analyzed paternal lineage in different Iranian ethnic groups, not only to identify developing medical etiology, but also to pave the way for gender-specific targeted strategies and personalized medicine in medical genetic research studies.
The diversity of eleven Iranian ethnic groups was studied using 27 Y-chromosomal short tandem repeat (Y-STR) haplotypes from Y-filer® Plus kit. Analysis of molecular variance (AMOVA) based on pair-wise R along with multidimensional scaling (MDS) calculation and Network phylogenic analysis was employed to quantify the differences between 503 unrelated individuals from each ethnicity.
Results from AMOVA calculation confirmed that Gilaks and Azeris showed the largest genetic distance (R=0.35434); however, Sistanis and Lurs had the smallest considerable genetic distance (R=0.00483) compared to other ethnicities. Although Azeris had a considerable distance from other ethnicities, they were still close to Turkmens. MDS analysis of ethnic groups gave the indication of lack of similarity between different ethnicities. Besides, network phylogenic analysis demonstrated insignificant clustering between samples.
The AMOVA analysis results explain that the close distance of Azeris and Turkmens may be the effect of male-dominant expansions across Central Asia that contributed to historical and demographics of populations in the region. Insignificant differences in network analysis could be the consequence of high mutation events that happened in the Y-STR regions over the years. Considering the ethnic group affiliations in medical research, our results provided an understanding and characterization of Iranian male population for future medical and population genetics studies.
Y染色体变异的研究为了解男性在某些疾病（如心血管疾病，CVD）中的易感性提供了有价值的见解。在本研究中，我们分析了不同伊朗族群的父系血统，不仅是为了确定疾病发生的医学病因，也是为了在医学遗传学研究中为针对性别的靶向策略和个性化医疗铺平道路。
使用来自Y-filer® Plus试剂盒的27个Y染色体短串联重复序列（Y-STR）单倍型，研究了11个伊朗族群的多样性。基于成对R的分子方差分析（AMOVA）以及多维尺度分析（MDS）计算和网络系统发育分析，用于量化来自每个族群的503名无关个体之间的差异。
AMOVA计算结果证实，吉拉克人和阿塞拜疆人显示出最大的遗传距离（R = 0.35434）；然而，与其他族群相比，锡斯坦人和卢尔人之间的遗传距离最小（R = 0.00483）。尽管阿塞拜疆人与其他族群有相当大的距离，但他们仍然与土库曼人接近。族群的MDS分析表明不同族群之间缺乏相似性。此外，网络系统发育分析表明样本之间的聚类不显著。
AMOVA分析结果表明，阿塞拜疆人和土库曼人之间的近距离可能是中亚男性主导扩张的结果，这对该地区的人口历史和人口统计学产生了影响。网络分析中不显著的差异可能是多年来Y-STR区域发生的高突变事件的结果。考虑到医学研究中的族群归属，我们的结果为未来的医学和群体遗传学研究提供了对伊朗男性群体的理解和特征描述。
