Lema F, Roseto A, Duverlie G, Peries J, Daniel Ph
Service de Virologie, Centre Hospitalier Universitaire, 80 000 Amiens, France.
Unité 107 INSERM, Hôpital Saint-Louis, 75010 Paris, France.
Med Mal Infect. 1981 Nov;11(11):576-580. doi: 10.1016/S0399-077X(81)80043-1. Epub 2005 May 26.
A systematic survey was carried on stools from 130 children suffering of acute gastroenteritis. Electron microscopy, enzymo-linked immunosorbent assay (ELISA) and counter electrophoresis were employed. This survey allowed to the detection by electron microscopy of Rotavirus (40 cases), Coronaviruses (3 cases), Astroviruses (2 cases), Adenoviruses (2 cases) and Small Round Viruses (1 case). Serological tests (complement fixation, ELISA and counter electrophoresis) done with 86 sera showed a good correlation with results obtained with electron microscopy.
对130名患急性肠胃炎儿童的粪便进行了系统调查。采用了电子显微镜检查、酶联免疫吸附测定(ELISA)和对流免疫电泳。通过电子显微镜检查发现了轮状病毒(40例)、冠状病毒(3例)、星状病毒(2例)、腺病毒(2例)和小圆小圆状病毒(1例)。对86份血清进行的血清学检测(补体结合试验、ELISA和对流免疫电泳)结果与电子显微镜检查结果具有良好的相关性。