Dei R, Urbano P, Franceschini E, Bartolozzi G, De Mayo E
Boll Ist Sieroter Milan. 1980 May 31;59(2):159-65.
We report the use of the line immunoelectroosmophoresis (LIEOP), a modification of the immunoelectroosmophoresis (IEOP), for the serodiagnosis of rotavirus infection, in comparison with the complement fixation test. We tested 17 paired and 29 single sera from hospitalized children affected by acute gastroenteritis; rotavirus had been searched in the stools both by electron microscopy and IEOP. The LIEOP showed seroconversion in all the 11 cases with positive stools and also in 5 out of 5 patients whose stool sample had been negative. Either we had not been able to detect the virus, or there was an intervening (nosocomial) rotavirus infection. All sera which were reactive by complement fixation with bovine rotavirus were also reactive by LIEOP. Some of the sera which reacted by LIEOP, both with the homologous and the heterologous antigen, were negative by complement fixation.
我们报告了使用线免疫电渗电泳（LIEOP）（免疫电渗电泳（IEOP）的一种改良方法）进行轮状病毒感染的血清学诊断，并与补体结合试验进行了比较。我们检测了17对以及29份来自患急性肠胃炎住院儿童的单份血清；通过电子显微镜和IEOP在粪便中检测轮状病毒。LIEOP在所有粪便检测呈阳性的11例病例中均显示血清转化，在粪便样本检测为阴性的5例患者中的5例也显示血清转化。要么是我们未能检测到病毒，要么是存在介入性（医院内）轮状病毒感染。所有通过补体结合试验与牛轮状病毒发生反应的血清，通过LIEOP也呈反应性。一些通过LIEOP与同源和异源抗原均发生反应的血清，补体结合试验结果为阴性。
