Konno T, Suzuki H, Imai A, Ishida N
J Infect Dis. 1977 Feb;135(2):259-66. doi: 10.1093/infdis/135.2.259.
The reovirus-like agent, sometimes referred to as duovirus or rotavirus, was visualized by electron microscopy in stool extracts from Japanese infants and young children with acute epidemic gastroenteritis. The virus particles measured 70 nm in diameter and had double-shelled capsids. One hundred ten (89%) of 124 patients with the gastroenteritis had such virus particles in stools obtained during the acute phase. The virus particles were excreted in the stools usually during the first eight days of illness. Agglutination of virus particles by antibody present in convalescent-phase sera was demonstrated by immune electron microscopy. Complement-fixing antibody was detected as early as day 3 of illness, and antibody titers peaked during the second and third weeks of the disease. The antibody appearing in the acute and early convalescent phases was sensitive to 2-mercaptoethanol. Antibody resistant to 2-mercaptoethanol was produced approximately 10 days after the onset of the symptoms. The serologic evidence suggests that a primary infection with the reovirus-like agent was responsible for the clinical attack of acute gastroenteritis.
呼肠孤病毒样病原体，有时被称为双病毒或轮状病毒，通过电子显微镜在患有急性流行性肠胃炎的日本婴幼儿粪便提取物中被观察到。病毒颗粒直径为70纳米，具有双层衣壳。124例肠胃炎患者中有110例（89%）在急性期采集的粪便中含有此类病毒颗粒。病毒颗粒通常在发病的前八天随粪便排出。通过免疫电子显微镜证实了恢复期血清中的抗体可使病毒颗粒发生凝集。早在发病第3天就检测到补体结合抗体，抗体滴度在疾病的第二和第三周达到峰值。在急性期和恢复期早期出现的抗体对2-巯基乙醇敏感。症状出现约10天后产生对2-巯基乙醇有抗性的抗体。血清学证据表明，初次感染呼肠孤病毒样病原体是急性肠胃炎临床发作的原因。
