Department of Mycology and Genetics, University of Wrocław, Przybyszewskiego Str 63/77, Wrocław, Poland.
Braz J Microbiol. 2024 Jun;55(2):1793-1800. doi: 10.1007/s42770-024-01321-1. Epub 2024 Apr 16.
Trichosporon spp. is an emerging opportunistic pathogen and a common cause of both superficial and invasive infections. Although Trichosporon asahii is the most frequently isolated species, Trichosporon cutaneum is also widely observed, as it is the predominant agent in cases of white Piedra and onychomycosis. Trichosporon spp. is a known to produce biofilms, which serve as one of its virulence mechanisms, however, there is limited data available on biofilms formed by T. cutaneum. Thus, the aim of this study was to assess the adhesion and biofilm formation of two clinical isolates of T. cutaneum under various environmental conditions (including temperature, nutrient availability, and carbon source), as well as their tolerance to fluconazole. Adhesion was tested on common abiotic substrates (such as silicone, glass, and stainless steel), revealing that T. cutaneum readily adhered to all surfaces tested. CV staining was applied for the evaluation of the environment influence on biofilm efficiency and it was proved that the nutrient availability has a major impact. Additionaly, fluorescent staining was employed to visualize the morphology of T. cutaneum biofilm and its survival in the presence of fluconazole. Hyphae production was shown to play a role in elevated biofilm production in minimal medium and increased tolerance to fluconazole.
发状念珠菌属是一种新兴的机会致病菌，也是浅部和深部感染的常见原因。虽然近平滑假丝酵母是最常分离到的菌种，但须毛状假丝酵母也广泛存在，因为它是白色 Piedra 和甲真菌病的主要病原体。发状念珠菌属已知会产生生物膜，这是其毒力机制之一，然而，关于须毛状假丝酵母形成的生物膜的数据有限。因此，本研究旨在评估两种临床分离的须毛状假丝酵母在各种环境条件（包括温度、营养可用性和碳源）下的黏附与生物膜形成能力，以及它们对氟康唑的耐受性。黏附性在常见的非生物基质（如硅酮、玻璃和不锈钢）上进行测试，结果表明须毛状假丝酵母很容易黏附在所有测试的表面上。CV 染色用于评估环境对生物膜效率的影响，结果证明营养可用性有重大影响。此外，荧光染色用于可视化须毛状假丝酵母生物膜的形态及其在氟康唑存在下的存活情况。丝状菌的产生在最小培养基中增加了生物膜的产生，并提高了对氟康唑的耐受性。
