对从大肠杆菌突变体中分离出的tRNATyr反密码子中7-(氨甲基)-7-脱氮鸟苷进行特异性荧光标记。
Specific fluorescent labeling of 7-(aminomethyl)-7-deazaguanosine located in the anticodon of tRNATyr isolated from E. coli mutant.
作者信息
Kasai H, Shindo-Okada N, Noguchi S, Nishimura S
出版信息
Nucleic Acids Res. 1979 Sep 11;7(1):231-8. doi: 10.1093/nar/7.1.231.
Abstract
Under-modified E. coli tRNATyr that contains 7-(aminomethyl)-7-deazaguanosine in place of Q nucleoside can be chemically modified by dansyl chloride under neutral conditions. Fluorescent labelling specifically occurred only in the 7-(aminomethyl)-7-deazaguanine moiety. The modified tRNATyr was found to be active both in aminoacylation and in binding to ribosomes.
摘要
含有7-(氨甲基)-7-脱氮鸟苷取代Q核苷的未充分修饰的大肠杆菌tRNATyr可以在中性条件下用丹磺酰氯进行化学修饰。荧光标记仅特异性地发生在7-(氨甲基)-7-脱氮鸟嘌呤部分。发现修饰后的tRNATyr在氨酰化和与核糖体结合方面均具有活性。
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