荧光素硫代氨基甲酰 - tRNATyr（大肠杆菌）：一种用于物理化学研究的tRNATyr（大肠杆菌）的有用衍生物。
Fluoresceinylthiocarbamyl-tRNATyr: a useful derivative of tRNATyr (E.coli) for physicochemical studies.
作者信息
Pingoud A, Kownatzki R, Maass G
出版信息
Nucleic Acids Res. 1977 Feb;4(2):327-38. doi: 10.1093/nar/4.2.327.
Abstract
Fluoresceinylthiocarbamyl-tRNATyr (FTC-tRNATyr) is prepared from tRNATyr and fluoresceinisothiogyanate (FITC) under mildly alkaline conditions. Labelling occurs specificly at the base Q of tRNATyr. The modified tRNA is fully active in the aminoacylation assay; when aminoacylated it is recognized by the elongation factor Tu (EF-Tu). Codon-anticodon interaction, however, is severely affected by the modification.
摘要
荧光素硫代氨基甲酰 - tRNATyr（FTC - tRNATyr）是在温和碱性条件下由tRNATyr和异硫氰酸荧光素（FITC）制备而成。标记特异性地发生在tRNATyr的碱基Q处。修饰后的tRNA在氨酰化测定中具有完全活性；氨酰化后它能被延伸因子Tu（EF - Tu）识别。然而，密码子 - 反密码子相互作用受到修饰的严重影响。
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2
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3
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