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基于基因组学的方法评估从智利温泉中分离出的CamBx3的代谢潜力和胞外多糖产量。

Genome-based approach to evaluate the metabolic potentials and exopolysaccharides production of CamBx3 isolated from a Chilean hot spring.

作者信息

Narsing Rao Manik Prabhu, Singh Ram Nageena, Sani Rajesh K, Banerjee Aparna

机构信息

Instituto de Ciencias Aplicadas, Facultad de Ingeniería, Universidad Autónoma de Chile, Sede Talca, Talca, Chile.

Department of Chemical and Biological Engineering, South Dakota Mines, Rapid City, SD, United States.

出版信息

Front Microbiol. 2024 Apr 2;15:1377965. doi: 10.3389/fmicb.2024.1377965. eCollection 2024.

DOI:10.3389/fmicb.2024.1377965
PMID:38628868
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11018918/
Abstract

In the present study, a thermophilic strain designated CamBx3 was isolated from the Campanario hot spring, Chile. Based on 16S rRNA gene sequence, phylogenomic, and average nucleotide identity analysis the strain CamBx3 was identified as . Genome analysis of CamBx3 revealed the presence of genes related to heat tolerance, exopolysaccharides (EPS), dissimilatory nitrate reduction, and assimilatory sulfate reduction. The pangenome analysis of strain CamBx3 with eight spp. resulted in 26,562 gene clusters, 7,002 shell genes, and 19,484 cloud genes. The EPS produced by CamBx3 was extracted, partially purified, and evaluated for its functional activities. CamBx3 EPS with concentration 5 mg mL showed an optimum 92 mM ferrous equivalent FRAP activity, while the same concentration showed a maximum 91% of Fe chelating activity. CamBx3 EPS (0.2 mg mL) demonstrated -glucosidase inhibition. The EPS formed a viscoelastic gel at 45°C with a maximum instantaneous viscosity of 315 Pa.s at acidic pH 5. The present study suggests that CamBx3 could be a valuable resource for biopolymers and bioactive molecules for industrial applications.

摘要

在本研究中，从智利坎帕纳里奥温泉中分离出一株嗜热菌株，命名为CamBx3。基于16S rRNA基因序列、系统基因组学和平均核苷酸同一性分析，菌株CamBx3被鉴定为[具体名称未给出]。对CamBx3的基因组分析表明，其存在与耐热性、胞外多糖（EPS）、异化硝酸盐还原和同化硫酸盐还原相关的基因。对菌株CamBx3与8种[具体物种未给出]进行泛基因组分析，结果得到26,562个基因簇、7,002个核心基因和19,484个可变基因。提取、部分纯化了CamBx3产生的EPS，并对其功能活性进行了评估。浓度为5 mg/mL的CamBx3 EPS表现出最佳的92 mM亚铁当量的铁还原抗氧化能力（FRAP）活性，而相同浓度的EPS表现出最大91%的铁螯合活性。CamBx3 EPS（0.2 mg/mL）表现出对α-葡萄糖苷酶的抑制作用。该EPS在45°C、酸性pH 5条件下形成粘弹性凝胶，最大瞬时粘度为315 Pa·s。本研究表明，CamBx3可能是用于工业应用的生物聚合物和生物活性分子的宝贵资源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a86/11018918/30d67a190a39/fmicb-15-1377965-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a86/11018918/13a2fe5dcca7/fmicb-15-1377965-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a86/11018918/e05b162264be/fmicb-15-1377965-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a86/11018918/0f0ba11e4a9a/fmicb-15-1377965-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a86/11018918/bb323b3b8faf/fmicb-15-1377965-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a86/11018918/62ca067b8f66/fmicb-15-1377965-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a86/11018918/30d67a190a39/fmicb-15-1377965-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a86/11018918/13a2fe5dcca7/fmicb-15-1377965-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a86/11018918/e05b162264be/fmicb-15-1377965-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a86/11018918/0f0ba11e4a9a/fmicb-15-1377965-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a86/11018918/bb323b3b8faf/fmicb-15-1377965-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a86/11018918/62ca067b8f66/fmicb-15-1377965-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1a86/11018918/30d67a190a39/fmicb-15-1377965-g006.jpg

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