Lippel K, Olson J A
J Lipid Res. 1968 Sep;9(5):580-6.
After the intraportal injection of retinoic acid-15-(14)C into rats, all-trans methyl retinoate, a cis isomer of methyl retinoate, retinoyl beta-glucurono-gamma-lactone, retinoic acid, and retinoyl beta-glucuronide were isolated from methanol extracts of rat bile by chromatography on anion-exchange resin and silicic acid columns and characterized on thin-layer plates of Silica Gel G. On the other hand, when bile was extracted with n-butanol or analyzed directly by thin-layer chromatography, only retinoyl beta-glucuronide and a very small amount of retinoic acid could be detected. Butanol extracts of the liver and the intestine, however, still contained a small radioactive nonpolar fraction. When retinoyl beta-glucuronide was incubated with an anion-exchange resin in the presence of methanol, several nonpolar products appeared. Apparently the methyl retinoate, retinoyl beta-glucurono-gamma-lactone, and most of the retinoic acid previously found in bile after retinoic acid administration are produced from retinoyl beta-glucuronide during the isolation procedure.
给大鼠门静脉注射视黄酸 -15-(14)C 后，通过阴离子交换树脂柱和硅酸柱色谱法从大鼠胆汁的甲醇提取物中分离出全反式甲基视黄酸、甲基视黄酸的顺式异构体、视黄酰β - 葡糖醛酸 -γ - 内酯、视黄酸和视黄酰β - 葡糖醛酸，并在硅胶G薄板上进行表征。另一方面，当用正丁醇提取胆汁或直接通过薄层色谱分析时，仅能检测到视黄酰β - 葡糖醛酸和极少量的视黄酸。然而，肝脏和肠道的丁醇提取物仍含有少量放射性非极性部分。当视黄酰β - 葡糖醛酸在甲醇存在下与阴离子交换树脂一起孵育时，出现了几种非极性产物。显然，视黄酸给药后先前在胆汁中发现的甲基视黄酸、视黄酰β - 葡糖醛酸 -γ - 内酯和大部分视黄酸是在分离过程中由视黄酰β - 葡糖醛酸产生的。
