Caruthers M H
Science. 1985 Oct 18;230(4723):281-5. doi: 10.1126/science.3863253.
Deoxyoligonucleotides can now be synthesized rapidly and in high yield because of recent advances in nucleic acid chemistry. Key innovations include solid-phase synthesis on silica-based supports and the development of stable deoxynucleoside phosphoramidites as synthons. When incorporated into manual, semiautomatic, or automatic instruments, these new procedures can be used to prepare probes, mixed probes, deoxyoligonucleotides for priming DNA synthesis, analogues of deoxyoligonucleotides, and DNA segments containing more than 100 deoxynucleotides.
由于核酸化学的最新进展，脱氧寡核苷酸现在可以快速且高产率地合成。关键创新包括基于二氧化硅载体的固相合成以及稳定的脱氧核苷亚磷酰胺作为合成子的开发。当与手动、半自动或自动仪器结合使用时，这些新方法可用于制备探针、混合探针、用于引发DNA合成的脱氧寡核苷酸、脱氧寡核苷酸类似物以及含有超过100个脱氧核苷酸的DNA片段。
