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通过工程改造毕赤酵母从甲醇中微生物合成长链α-烯烃

Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris.

作者信息

Cai Peng, Li Yunxia, Zhai Xiaoxin, Yao Lun, Ma Xiaojun, Jia Lingyun, Zhou Yongjin J

机构信息

School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, People's Republic of China.

Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, People's Republic of China.

出版信息

Bioresour Bioprocess. 2022 May 26;9(1):58. doi: 10.1186/s40643-022-00551-1.

DOI:10.1186/s40643-022-00551-1
PMID:38647822
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10991524/
Abstract

α-Alkenes (terminal alkenes) are important fuel and platform chemicals that are mainly produced from petroleum. Microbial synthesis might provide a sustainable approach for α-alkenes. In this work, we engineered the methylotrophic yeast Pichia pastoris to produce long-chain (C15:1, C17:1 and C17:2) α-alkenes via a decarboxylation of fatty acids. Combinatorial engineering, including enzyme selection, expression optimization and peroxisomal compartmentalization, enabled the production of 1.6 mg/L α-alkenes from sole methanol. This study represents the first case of α-alkene biosynthesis from methanol and also provides a reference for the construction of methanol microbial cell factories of other high-value chemicals.

摘要

α-烯烃（末端烯烃）是重要的燃料和平台化学品，主要由石油生产。微生物合成可能为α-烯烃提供一种可持续的方法。在这项工作中，我们对甲基营养型酵母毕赤酵母进行了工程改造，使其通过脂肪酸脱羧作用生产长链（C15:1、C17:1和C17:2）α-烯烃。包括酶选择、表达优化和过氧化物酶体区室化在内的组合工程，使得仅从甲醇就能生产出1.6毫克/升的α-烯烃。本研究代表了从甲醇生物合成α-烯烃的首例，也为构建其他高价值化学品的甲醇微生物细胞工厂提供了参考。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60ef/10991524/7a2b3baa3b96/40643_2022_551_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60ef/10991524/1b5f99d2e21f/40643_2022_551_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60ef/10991524/00acc29896d4/40643_2022_551_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60ef/10991524/be204c51a893/40643_2022_551_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60ef/10991524/7a2b3baa3b96/40643_2022_551_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60ef/10991524/1b5f99d2e21f/40643_2022_551_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60ef/10991524/00acc29896d4/40643_2022_551_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60ef/10991524/be204c51a893/40643_2022_551_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/60ef/10991524/7a2b3baa3b96/40643_2022_551_Fig4_HTML.jpg

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