Dani Sophie, Schütz Kathleen, Dikici Ezgi, Bernhardt Anne, Lode Anja
Centre for Translational Bone, Joint and Soft Tissue Research, Faculty of Medicine, Technical University Dresden, Dresden, Germany.
Sci Rep. 2024 Apr 24;14(1):9444. doi: 10.1038/s41598-024-60014-9.
One of the biggest challenges in tissue engineering and regenerative medicine is to ensure oxygen supply of cells in the (temporary) absence of vasculature. With the vision to exploit photosynthetic oxygen production by microalgae, co-cultivated in close vicinity to oxygen-consuming mammalian cells, we are searching for culture conditions that are compatible for both sides. Herein, we investigated the impact of long-term illumination on mammalian cells which is essential to enable photosynthesis by microalgae: four different cell types-primary human fibroblasts, dental pulp stem cells, and osteoblasts as well as the murine beta-cell line INS-1-were continuously exposed to warm white light, red or blue light over seven days. We observed that illumination with red light has no adverse effects on viability, metabolic activity and growth of the cells whereas exposure to white light has deleterious effects that can be attributed to its blue light portion. Quantification of intracellular glutathione did not reveal a clear correlation of this effect with an enhanced production of reactive oxygen species. Finally, our data indicate that the cytotoxic effect of short-wavelength light is predominantly a direct effect of cell illumination; photo-induced changes in the cell culture media play only a minor role.
组织工程和再生医学面临的最大挑战之一是在（暂时）没有血管系统的情况下确保细胞的氧气供应。为了利用与消耗氧气的哺乳动物细胞近距离共培养的微藻进行光合作用产氧，我们正在寻找对双方都兼容的培养条件。在此，我们研究了长期光照对哺乳动物细胞的影响，这对于微藻进行光合作用至关重要：四种不同的细胞类型——原代人成纤维细胞、牙髓干细胞、成骨细胞以及小鼠β细胞系INS-1——连续七天暴露于暖白光、红光或蓝光下。我们观察到，红光照射对细胞的活力、代谢活性和生长没有不利影响，而暴露于白光则有有害影响，这可归因于其蓝光部分。细胞内谷胱甘肽的定量分析并未揭示这种效应与活性氧生成增加之间存在明显相关性。最后，我们的数据表明，短波长光的细胞毒性作用主要是细胞光照的直接效应；细胞培养基中光诱导的变化只起次要作用。
