Rogalski T M, Baillie D L
Mol Gen Genet. 1985;201(3):409-14. doi: 10.1007/BF00331331.
The genetic organization of the region immediately adjacent to the unc-22 IV gene in Caenorhabditis elegans has been studied. We have identified twenty essential genes in this interval of approximately 1.5-map units on Linkage Group IV. The mutations that define these genes were positioned by recombination mapping and complementation with several deficiencies. With few exceptions, the positions obtained by these two methods agreed. Eight of the twenty essential genes identified are represented by more than one allele. Three possible internal deletions of the unc-22 gene have been located by intra-genic mapping. In addition, the right end point of a deficiency or an inversion affecting the adjacent genes let-56 and unc-22 has been positioned inside the unc-22 gene.
对秀丽隐杆线虫中紧邻unc-22 IV基因的区域的基因组织进行了研究。我们在连锁群IV上大约1.5个图距单位的这个区间内鉴定出了20个必需基因。通过重组作图和与几个缺失突变体互补定位了定义这些基因的突变。除了少数例外，通过这两种方法获得的位置是一致的。所鉴定出的20个必需基因中有8个由不止一个等位基因代表。通过基因内作图定位了unc-22基因的三种可能的内部缺失。此外，一个影响相邻基因let-56和unc-22的缺失或倒位的右端点已定位在unc-22基因内部。
