Rogalski T M, Moerman D G, Baillie D L
Genetics. 1982 Dec;102(4):725-36. doi: 10.1093/genetics/102.4.725.
Five formaldehyde-induced deficiencies that uncover unc-22 IV, a gene affecting muscle structure in the nematode Caenorhabditis elegans were isolated and positioned. The largest deficiency, sDf2, extends in both directions from unc-22 and is approximately 1.0-2.0 map units in length. The other four deficiencies, sDf7, sDf8, sDf9 and sDf10, are all smaller than sDf2 and are located within the region uncovered by this deficiency. Thirty-seven ethyl methane-sulfonate-induced lethal and sterile mutations linked to unc-22 were isolated and tested for complementation with sDf2. Nineteen lethal mutations failed to complement sDf2. Sixteen of these were further positioned by recombination mapping and also by deficiency mapping with sDf7, sDf8, sDf9 and sDf10. These sixteen mutations define 11 new essential genes in this region. Eight of the genes lie in a 0.9-map unit interval to the left of unc-22, whereas the three remaining genes lie in a region of about 0.2 map units to the right of unc-22. We believe that two of the essential genes identified in this study, let-56 and let-52, are the adjacent genes on either side of unc-22. The lethal mutations exhibit a wide range of terminal phenotypes: from first stage larva to sterile adult.
分离并定位了五个由甲醛诱导产生的缺陷，这些缺陷揭示了unc-22 IV基因，该基因影响线虫秀丽隐杆线虫的肌肉结构。最大的缺陷sDf2从unc-22向两个方向延伸，长度约为1.0 - 2.0个图谱单位。其他四个缺陷sDf7、sDf8、sDf9和sDf10都比sDf2小，且位于该缺陷未覆盖的区域内。分离出37个与unc-22相关的由乙基甲磺酸诱导产生的致死和不育突变，并测试它们与sDf2的互补性。19个致死突变不能与sDf2互补。其中16个通过重组图谱定位，也通过与sDf7、sDf8、sDf9和sDf10的缺陷图谱定位。这16个突变在该区域定义了11个新的必需基因。其中8个基因位于unc-22左侧0.9个图谱单位的区间内，而其余3个基因位于unc-22右侧约0.2个图谱单位的区域内。我们认为本研究中鉴定的两个必需基因let-56和let-52是unc-22两侧的相邻基因。致死突变表现出广泛的终末表型：从第一期幼虫到不育成虫。
