Fedoreyeva Larisa Ivanovna, Lazareva Elena Michailovna, Kononenko Neonila Vasilievna
All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya 42, 127550 Moscow, Russia.
Biological Department, M.V. Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, Russia.
Int J Mol Sci. 2024 Apr 19;25(8):4487. doi: 10.3390/ijms25084487.
Hypoxia is one of the common abiotic stresses that negatively affects the development and productivity of agricultural crops. Quercetin is used to protect plants from oxidative stress when exposed to environmental stressors. O deficiency leads to impaired development and morphometric parameters in wheat varieties Orenburgskaya 22 ( L.) and varieties Zolotaya ( Desf.). Cytological analysis revealed various types of changes in the cytoplasm under conditions of hypoxia and treatment with quercetin. The most critical changes in the cytoplasm occur in the Zolotaya variety during pretreatment with quercetin followed by hypoxia, and in the Orenburgskaya 22 variety during hypoxia. Quercetin has a protective effect only on the Orenburgskaya 22 variety, and also promotes a more effective recovery after exposure to low O content. Hypoxia causes an increase in reactive oxygen species and activates the antioxidant system. It has been shown that the most active components of the antioxidant system in the Orenburgskaya 22 variety are MnSOD and Cu/ZnSOD, and in the Zolotaya variety GSH. We have shown that quercetin provides resistance only to the wheat genotype Orenburgskaya 22, as a protective agent against abiotic stress, which indicates the need for a comprehensive study of the effects of exogenous protectors before use in agriculture.
缺氧是一种常见的非生物胁迫，会对农作物的发育和生产力产生负面影响。槲皮素用于保护植物在暴露于环境胁迫时免受氧化应激。氧气缺乏会导致奥伦堡斯卡亚22号（L.）小麦品种和佐洛塔亚（Desf.）品种的发育和形态参数受损。细胞学分析揭示了在缺氧和槲皮素处理条件下细胞质的各种变化。细胞质中最关键的变化发生在佐洛塔亚品种中，即在槲皮素预处理后再进行缺氧处理时；而在奥伦堡斯卡亚22号品种中，则发生在缺氧期间。槲皮素仅对奥伦堡斯卡亚22号品种有保护作用，并且还能促进在低氧含量暴露后的更有效恢复。缺氧会导致活性氧增加并激活抗氧化系统。研究表明，奥伦堡斯卡亚22号品种中抗氧化系统最活跃的成分是锰超氧化物歧化酶（MnSOD）和铜/锌超氧化物歧化酶（Cu/ZnSOD），而在佐洛塔亚品种中是谷胱甘肽（GSH）。我们已经表明，槲皮素仅对小麦基因型奥伦堡斯卡亚22号有抗性，作为一种抗非生物胁迫的保护剂，这表明在农业中使用外源保护剂之前需要对其效果进行全面研究。
