State Key Laboratory for Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Rural Affairs and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, 315211, China.
National Key Laboratory of Wheat and Maize Crop Science/Collaborative Innovation Center of Henan Grain Crops/Agronomy College, Henan Agricultural University, Zhengzhou, 450000, China.
Nat Commun. 2022 Nov 2;13(1):6576. doi: 10.1038/s41467-022-34362-x.
Molecular manipulation of susceptibility (S) genes that are antipodes to resistance (R) genes has been adopted as an alternative strategy for controlling crop diseases. Here, we show the S gene encoding Triticum aestivum mA methyltransferase B (TaMTB) is identified by a genome-wide association study and subsequently shown to be a positive regulator for wheat yellow mosaic virus (WYMV) infection. TaMTB is localized in the nucleus, is translocated into the cytoplasmic aggregates by binding to WYMV NIb to upregulate the mA level of WYMV RNA1 and stabilize the viral RNA, thus promoting viral infection. A natural mutant allele TaMTB-SNP176C is found to confer an enhanced susceptibility to WYMV infection through genetic variation analysis on 243 wheat varieties. Our discovery highlights this allele can be a useful target for the molecular wheat breeding in the future.
通过对与抗性（R）基因相对立的易感性（S）基因进行分子操作，已被采纳为控制作物病害的一种替代策略。在这里，我们通过全基因组关联研究鉴定出编码 Triticum aestivum mA 甲基转移酶 B（TaMTB）的 S 基因，随后证明它是小麦黄花叶病毒（WYMV）感染的正向调节剂。TaMTB 定位于细胞核内，通过与 WYMV NIb 结合易位到细胞质聚集体中，上调 WYMV RNA1 的 mA 水平并稳定病毒 RNA，从而促进病毒感染。通过对 243 个小麦品种进行遗传变异分析，发现一个天然突变等位基因 TaMTB-SNP176C 可通过遗传变异分析赋予对 WYMV 感染的增强易感性。我们的发现强调了该等位基因可作为未来小麦分子育种的一个有用目标。
