牛结节性皮肤病病毒全基因组测序与分析揭示了西非和中非的一个新病毒亚群。

Sequencing and Analysis of Lumpy Skin Disease Virus Whole Genomes Reveals a New Viral Subgroup in West and Central Africa.

作者信息

Haga Ismar R, Shih Barbara B, Tore Gessica, Polo Noemi, Ribeca Paolo, Gombo-Ochir Delgerzul, Shura Gansukh, Tserenchimed Tsagaan, Enkhbold Bazarragchaa, Purevtseren Dulam, Ulziibat Gerelmaa, Damdinjav Batchuluun, Yimer Lama, Bari Fufa D, Gizaw Daniel, Adedeji Adeyinka Jeremy, Atai Rebecca Bitiyong, Adole Jolly Amoche, Dogonyaro Banenat Bajehson, Kumarawadu Pradeep Lakpriya, Batten Carrie, Corla Amanda, Freimanis Graham L, Tennakoon Chandana, Law Andy, Lycett Samantha, Downing Tim, Beard Philippa M

机构信息

The Pirbright Institute, Ash Road, Pirbright, Woking GU24 0NF, UK.

The Roslin Institute, University of Edinburgh, Easter Bush Campus, Roslin, Midlothian EH25 9RG, UK.

出版信息

Viruses. 2024 Apr 3;16(4):557. doi: 10.3390/v16040557.

DOI:10.3390/v16040557

PMID:38675899

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11053774/

Abstract

Lumpy skin disease virus (LSDV) is a member of the capripoxvirus (CPPV) genus of the family. LSDV is a rapidly emerging, high-consequence pathogen of cattle, recently spreading from Africa and the Middle East into Europe and Asia. We have sequenced the whole genome of historical LSDV isolates from the Pirbright Institute virus archive, and field isolates from recent disease outbreaks in Sri Lanka, Mongolia, Nigeria and Ethiopia. These genome sequences were compared to published genomes and classified into different subgroups. Two subgroups contained vaccine or vaccine-like samples ("Neethling-like" clade 1.1 and "Kenya-like" subgroup, clade 1.2.2). One subgroup was associated with outbreaks of LSD in the Middle East/Europe (clade 1.2.1) and a previously unreported subgroup originated from cases of LSD in west and central Africa (clade 1.2.3). Isolates were also identified that contained a mix of genes from both wildtype and vaccine samples (vaccine-like recombinants, grouped in clade 2). Whole genome sequencing and analysis of LSDV strains isolated from different regions of Africa, Europe and Asia have provided new knowledge of the drivers of LSDV emergence, and will inform future disease control strategies.

摘要

结节性皮肤病病毒（LSDV）是痘病毒科山羊痘病毒属的成员。LSDV是一种迅速出现的、对牛具有高后果性的病原体，最近已从非洲和中东传播到欧洲和亚洲。我们对来自皮尔布赖特研究所病毒库的历史LSDV分离株以及来自斯里兰卡、蒙古、尼日利亚和埃塞俄比亚近期疾病暴发的现场分离株进行了全基因组测序。将这些基因组序列与已发表的基因组进行比较，并分类为不同的亚组。两个亚组包含疫苗或类似疫苗的样本（“内斯林样”分支1.1和“肯尼亚样”亚组，分支1.2.2）。一个亚组与中东/欧洲的LSD暴发有关（分支1.2.1），一个先前未报告的亚组源自西非和中非的LSD病例（分支1.2.3）。还鉴定出了包含野生型和疫苗样本基因混合的分离株（类似疫苗的重组体，归类在分支2中）。对从非洲、欧洲和亚洲不同地区分离的LSDV毒株进行全基因组测序和分析，为LSDV出现的驱动因素提供了新知识，并将为未来的疾病控制策略提供信息。