Dashore Shuken, Chouhan Kavish, Nanda Soni, Sharma Aseem
Dr Dashore's DHL Centre, Vijay Nagar, Indore, Madhya Pradesh, India.
Consultant Dermatologist and Hair Transplant Surgeon, DERMACLINIX, E13 Defence Colony, Delhi, India.
Indian Dermatol Online J. 2021 Nov 25;12(Suppl 1):S12-S23. doi: 10.4103/idoj.idoj_269_21. eCollection 2021 Nov.
The goal of these recommendations is to provide a framework to practitioners for implementing useful, evidence-based recommendations for the preparation of platelet-rich plasma (PRP) in various dermatological indications. The Indian Association of Dermatologists, Venereologists and Leprologists (IADVL) assigned the task of preparing these recommendations to its task force on PRP. A comprehensive literature search was done in the English language on the preparation of PRP across multiple databases. The grade of evidence and strength of recommendation was evaluated on the GRADE framework (Grading of Recommendation, Assessment, Development and Evaluation). A draft of clinical recommendations was developed on the best available evidence which was also scrutinized and critically evaluated by the IADVL Academy of Dermatology. Based on the inputs received, this final consensus statement was prepared. A total of 45 articles (meta-analyses, prospective and retrospective studies, reviews [including chapters in books], and case series) were critically evaluated and the evidence thus gathered was used in the preparation of these recommendations. This expert group recommends the use of double-spin manual method for the preparation of PRP. The recommended parameters for centrifuge are 100-300 g for 5-10 min for the first spin and 400-700 g for 10-17 min for the second spin. The recommended platelet concentration in PRP for the treatment of various dermatological conditions is 1-1.5 million platelets/μL. The activation of PRP is not required when it is injected into soft tissues.
这些建议的目标是为从业者提供一个框架，以便在各种皮肤病适应症中实施关于制备富血小板血浆（PRP）的实用、循证建议。印度皮肤科、性病科和麻风病科医生协会（IADVL）将制定这些建议的任务交给了其PRP特别工作组。针对PRP的制备在多个数据库中进行了全面的英文文献检索。依据GRADE框架（推荐分级、评估、制定和评价）对证据等级和推荐强度进行了评估。根据现有最佳证据制定了临床建议草案，该草案也经过了IADVL皮肤病学学会的审查和严格评估。基于收到的意见，制定了这份最终的共识声明。总共对45篇文章（荟萃分析、前瞻性和回顾性研究、综述[包括书籍章节]以及病例系列）进行了严格评估，并将由此收集的证据用于制定这些建议。该专家组建议采用双离心手工法制备PRP。离心机的推荐参数为：第一次离心100 - 300 g，持续5 - 10分钟；第二次离心400 - 700 g，持续10 - 17分钟。用于治疗各种皮肤病的PRP中推荐的血小板浓度为100 - 150万个血小板/微升。当将PRP注入软组织时，无需对其进行激活。
