代谢组学和转录组学揭示了不同生境中Phlomoides rotata 中类黄酮生物合成和调控机制。

Metabolomic and transcriptomic reveal flavonoid biosynthesis and regulation mechanism in Phlomoides rotata from different habitats.

机构信息

Key Laboratory of Tibetan Plateau Medicinal Plant and Animal Resources, School of Life Sciences, Qinghai Normal University, Xining 810008, China.

Academy of Agricultural and Forestry Sciences, Qinghai University, Xining 810016, China.

出版信息

Genomics. 2024 May;116(3):110850. doi: 10.1016/j.ygeno.2024.110850. Epub 2024 Apr 27.

DOI:10.1016/j.ygeno.2024.110850

PMID:38685286

Abstract

Phlomoides rotata is a traditional medical plant at 3100-5200 m altitude in the Tibet Plateau. In this study, flavonoid metabolites were investigated in P. rotata from Henan County (HN), Guoluo County (GL), Yushu County (YS), and Chengduo County (CD) habitats in Qinghai. The level of kaempferol 3-neohesperidoside, sakuranetin, and biochanin A was high in HN. The content of limocitrin and isoquercetin was high in YS. The levels of ikarisoside A and chrysosplenol D in GL were high. Schaftoside, miquelianin, malvidin chloride, and glabrene in CD exhibited high levels. The results showed a significant correlation between 59 flavonoids and 29 DEGs. Eleven flavonoids increased with altitude. PAL2, UFGT6, COMT1, HCT2, 4CL4, and HCT3 genes were crucial in regulating flavonoid biosynthesis. Three enzymes CHS, 4CL, and UFGT, were crucial in regulating flavonoid biosynthesis. This study provided biological and chemical evidence for the different uses of various regional plants of P. rotata.

摘要

旋覆花是青藏高原海拔 3100-5200 米处的传统药用植物。本研究调查了来自河南（HN）、果洛（GL）、玉树（YS）和称多（CD）四个生境的旋覆花中的类黄酮代谢物。HN 中的山柰酚 3-新橙皮糖苷、樱花素和大豆苷元含量较高。YS 中的 limocitrin 和异槲皮苷含量较高。GL 中的 ikarisoside A 和 chrysosplenol D 含量较高。CD 中的 schaftoside、miquelianin、氯化锦葵色素和 glabrene 含量较高。结果表明，59 种类黄酮与 29 个 DEGs 之间存在显著相关性。11 种类黄酮随海拔升高而增加。PAL2、UFGT6、COMT1、HCT2、4CL4 和 HCT3 基因在调控类黄酮生物合成中起关键作用。CHS、4CL 和 UFGT 三种酶在调控类黄酮生物合成中起关键作用。本研究为不同地区旋覆花的不同用途提供了生物学和化学证据。