Miyasaki K T, Wilson M E, Zambon J J, Genco R J
Arch Oral Biol. 1985;30(11-12):843-8. doi: 10.1016/0003-9969(85)90141-4.
Actinobacillus actinomycetemcomitans and the genetically-related oral haemophili (Haemophilus segnis, Haemophilus aprhophilus and Haemophilus paraphrophilus) exhibit a range of sensitivities to the lethal effect of hydrogen peroxide (H2O2), A. actinomycetemcomitans being the most resistant. To extend this information, susceptibility to a range of H2O2 concentrations (10(-6)-10(-3) M) was assessed by incubating bacterial suspensions for 1 h at 37 degrees C in the presence of H2O2 and spreading the suspensions on chocolate agar plates to determine the concentration of H2O2 producing a 50 per cent reduction in colony-forming units (LD50). Catalase activity was quantified with a Clark-type oxygen electrode, which polarographically monitored the formation of dissolved oxygen in bacterial suspensions or sonicates following addition of reagent H2O2. Sensitivity to H2O2 did not correlate with catalase activity, either in intact cells or in bacterial sonicates. Specifically, some bacterial strains with undetectable catalase activity were highly resistant to H2O2. Micromolar concentrations of sodium azide which completely inhibited cell-associated catalase activity did not affect the resistance of A. actinomycetemcomitans to H2O2. Thus, the endogenous catalase activity of A. actinomycetemcomitans and certain oral haemophili is not an important determinant of resistance to the bactericidal effects of H2O2.
伴放线放线杆菌以及与之有基因关联的口腔嗜血菌（迟钝嗜血杆菌、嗜aphophilus嗜血杆菌和副嗜沫嗜血杆菌）对过氧化氢（H2O2）的致死作用表现出一系列敏感性，伴放线放线杆菌最为耐药。为了扩展这方面的信息，通过在37℃下将细菌悬液在H2O2存在下孵育1小时，并将悬液涂布在巧克力琼脂平板上以确定产生50%集落形成单位减少（LD50）的H2O2浓度，评估了对一系列H2O2浓度（10^(-6)-10^(-3)M）的敏感性。用过氧化氢酶活性用Clark型氧电极进行定量，该电极在加入试剂H2O2后极谱监测细菌悬液或超声裂解物中溶解氧的形成。无论是在完整细胞还是细菌超声裂解物中，对H2O2的敏感性都与过氧化氢酶活性无关。具体而言，一些过氧化氢酶活性检测不到的细菌菌株对H2O2高度耐药。微摩尔浓度的叠氮化钠完全抑制细胞相关的过氧化氢酶活性，但不影响伴放线放线杆菌对H2O2的耐药性。因此，伴放线放线杆菌和某些口腔嗜血菌的内源性过氧化氢酶活性不是对H2O2杀菌作用耐药性的重要决定因素。
