Miyasaki K T, Wilson M E, Reynolds H S, Genco R J
Infect Immun. 1984 Dec;46(3):644-8. doi: 10.1128/iai.46.3.644-648.1984.
We compared the sensitivities of oral and nonoral isolates of Actinobacillus actinomycetemcomitans, Haemophilus segnis, H. aphrophilus, and H. paraphrophilus to the bactericidal action of reagent hydrogen peroxide (H2O2). Susceptibility to a range of H2O2 concentrations (10(-6) to 10(-3) M) was assessed by incubating bacterial suspensions for 1 h at 37 degrees C in the presence of H2O2 and plating on chocolate agar to determine the concentration of H2O2 that would produce a 50% reduction in CFU (50% lethal dose). As a group, A. actinomycetemcomitans was more resistant to H2O2 than the oral haemophili, and H. aphrophilus was much more sensitive than all other organisms tested. The range of 50% lethal dose values for A. actinomycetemcomitans was between 8.5 X 10(-5) and 10(-3) M H2O2 or above. In contrast, H. aphrophilus exhibited 50% lethal dose values from below 1 X 10(-6) to 3.4 X 10(-4) M H2O2. The resistance of A. actinomycetemcomitans to H2O2 may be sufficient to protect these organisms from direct H2O2-mediated killing by host phagocytes.
我们比较了伴放线放线杆菌、迟钝嗜血杆菌、嗜沫嗜血杆菌和副嗜沫嗜血杆菌的口腔分离株和非口腔分离株对试剂过氧化氢（H2O2）杀菌作用的敏感性。通过在37℃下于H2O2存在的条件下将细菌悬液孵育1小时，并接种于巧克力琼脂平板以确定能使菌落形成单位（CFU）减少50%的H2O2浓度（50%致死剂量），来评估对一系列H2O2浓度（10^(-6)至10^(-3)M）的敏感性。总体而言，伴放线放线杆菌比口腔嗜血杆菌对H2O2更具抗性，且嗜沫嗜血杆菌比所有其他受试生物更敏感。伴放线放线杆菌的50%致死剂量值范围在8.5×10^(-5)至10^(-3)M H2O2或更高。相比之下，嗜沫嗜血杆菌的50%致死剂量值范围为低于1×10^(-6)至3.4×10^(-4)M H2O2。伴放线放线杆菌对H2O2的抗性可能足以保护这些生物体免受宿主吞噬细胞直接由H2O2介导的杀伤。
